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Enzymatic saccharification of pretreated wheat straw: Comparison of solids-recycling, sequential hydrolysis and batch hydrolysis

机译:预处理麦秸的酶促糖化:固体回收,顺序水解和间歇水解的比较

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摘要

In the enzymatic hydrolysis of lignocellulose materials, the recycling of the solid residue has previously been considered within the context of enzyme recycling. In this study, a steady state investigation of a solids-recycling process was made with pretreated wheat straw and compared to sequential and batch hydrolysis at constant reaction times, substrate feed and liquid and enzyme consumption. Compared to batch hydrolysis, the recycling and sequential processes showed roughly equal hydrolysis yields, while the volumetric productivity was significantly increased. In the 72 h process the improvement was 90% due to an increased reaction consistency, while the solids feed was 16% of the total process constituents. The improvement resulted primarily from product removal, which was equally efficient in solidsrecycling and sequential hydrolysis processes. No evidence of accumulation of enzymes beyond the accumulation of the substrate was found in recycling. A mathematical model of solids-recycling was constructed, based on a geometrical series.
机译:在木质纤维素材料的酶促水解中,先前已经在酶循环的背景下考虑了固体残余物的循环。在这项研究中,对经过预处理的麦秸进行了固体回收过程的稳态研究,并将其与在恒定反应时间,底物进料以及液体和酶的消耗下连续水解和间歇水解进行了比较。与分批水解相比,再循环和顺序过程显示出大致相等的水解收率,而容积生产率显着提高。在72小时的过程中,由于提高了反应的一致性,因此改善了90%,而固体进料占了整个过程成分的16%。改善主要来自产品去除,这在固体回收和顺序水解过程中同样有效。在回收中没有发现酶积累超过底物积累的证据。基于几何级数,构建了固体回收的数学模型。

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