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Sugar production from barley straw biomass pretreated by combined alkali and enzymatic extrusion

机译:碱法和酶法联合预处理从大麦秸秆生物质制糖

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摘要

A pretreatment that combines a thermo-mechanical process (extrusion) with chemical and biological catalysts to produce fermentable sugars from barley straw (BS) biomass was investigated. BS was firstly extruded with alkali and then, the pretreated material (extrudate) was submitted to extrusion with hydrolytic enzymes (bioextrusion). The bioextrudate was found to have 35% (w/w dwb) of total solids in soluble form, partly coming from carbohydrate hydrolysis during bioextrusion. About 48% of soluble solids dry weight is comprised by sugars, mostly glucose and xylose. Further enzymatic hydrolysis of bioextrudate could be successfully carried out at high solid loading level of 30% (w/v), with sugar production yield of 32 g glucose and 18 g xylose/100 g bioextrudate at 72 h incubation (equivalent to 96 and 52 g/l concentration, respectively). These results, together with the high level of integration of the process, indicate a great potential of this pretreatment technology for sugar production from lignocellulosic substrates.
机译:研究了将热机械过程(挤压)与化学和生物催化剂相结合以从大麦秸秆(BS)生物质生产可发酵糖的预处理。 BS首先用碱挤压,然后将预处理的材料(挤压物)用水解酶挤压(生物挤压)。发现该生物挤出物具有可溶形式的总固体的35%(w / w dwb),部分来自生物挤出过程中的碳水化合物水解。糖占可溶性固形物干重的约48%,主要是葡萄糖和木糖。可以在30%(w / v)的高固体负载水平下成功地进行生物挤出物的进一步酶水解,在72小时的温育下,糖的产量为32 g葡萄糖和18 g木糖/ 100 g生物挤出物(相当于96和52) g / l浓度)。这些结果以及该方法的高集成度表明,这种预处理技术具有很大的潜力,可以从木质纤维素底物中生产糖。

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