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首页> 外文期刊>Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies >Biodiesel production from crude jatropha oil catalyzed by immobilized lipase/acyltransferase from Candida parapsilosis in aqueous medium
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Biodiesel production from crude jatropha oil catalyzed by immobilized lipase/acyltransferase from Candida parapsilosis in aqueous medium

机译:固定化脂肪酶/酰基转移酶在水介质中催化副麻生念珠菌催化粗麻疯树油生产生物柴油

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摘要

The lipase/acyltransferase from Candida parapsilosis (CpLIP2) immobilized on two synthetic resins (Accurel MP 1000 and Lewatit VP OC 1600) was used as catalyst for the production of biodiesel (fatty acid methyl esters, FAME) by transesterification of jatropha oil with methanol, in a lipid/aqueous system. The oil was dispersed in a buffer solution (pH 6.5) containing methanol in excess (2 M in the biphasic system; molar ratio methanol/acyl chains 2: 1). Transesterification was carried out at 30 degrees C, under magnetic stirring, using 10% (w/w) of immobilized enzyme in relation to oil. The maximum FAME yields were attained after 8 h reaction time: 80.5% and 93.8%, when CpLIP2 immobilized on Accurel MP 1000 or on Lewatit VP OC 1600 were used, respectively. CpLIP2 on both Accurel MP 1000 and Lewatit VP OC 1600 showed high operational stability along 5 consecutive 8 h batches. (C) 2016 Elsevier Ltd. All rights reserved.
机译:固定在两种合成树脂(Accurel MP 1000和Lewatit VP OC 1600)上的副产假丝酵母(CpLIP2)的脂肪酶/酰基转移酶被用作催化剂,用于通过麻疯树油与甲醇的酯交换反应生产生物柴油(脂肪酸甲酯,FAME),在脂质/水系统中。将油分散在含有过量甲醇(双相体系中为2 M;甲醇/酰基链的摩尔比为2:1)的缓冲溶液(pH 6.5)中。酯交换反应是在30°C磁力搅拌下,相对于油使用10%(w / w)固定化酶进行的。在8小时的反应时间之后,当使用固定在Accurel MP 1000或Lewatit VP OC 1600上的CpLIP2时,最大的FAME产率达到了80.5%和93.8%。 Accurel MP 1000和Lewatit VP OC 1600上的CpLIP2在连续5个8小时批次中显示出很高的运行稳定性。 (C)2016 Elsevier Ltd.保留所有权利。

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