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首页> 外文期刊>Bioresource Technology: Biomass, Bioenergy, Biowastes, Conversion Technologies, Biotransformations, Production Technologies >Increasing the lignin yield of the Alkaline Polyol Pulping process by treating black liquor with laccases of Myceliophthora thermophila
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Increasing the lignin yield of the Alkaline Polyol Pulping process by treating black liquor with laccases of Myceliophthora thermophila

机译:通过用嗜热毁丝霉漆酶处理黑液来提高碱性多元醇制浆工艺的木质素收率

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摘要

The Alkaline Polyol Pulping process separates cellulose from lignocellulosic biomass by dissolving lignin to a great extent. Due to the pulping conditions the dissolved lignin depolymerises and only 75% can be precipitated. To increase this amount, a 24 h reaction of laccases of Myceliophthora thermophila with lignin dissolved in black liquor of the AlkaPolP process was investigated. The influence of pH, temperature, enzyme concentration and partial oxygen pressure was examined in a batch stirred tank reactor using a Box-Behnken factorial design. Due to the enzymatic reaction the lignin polymerises which results in an enhanced lignin precipitation. The addition of a mediator improves the polymerisation but decreases the amount of precipitable lignin. The influence of the parameters on precipitation yield and molecular mass can sufficiently be described with a second-order model and optimum conditions can be assessed. FT-IR spectra of the obtained lignins revealed that its typical phenolic structure is preserved. (C) 2015 Elsevier Ltd. All rights reserved.
机译:碱性多元醇制浆工艺通过在很大程度上溶解木质素将纤维素与木质纤维素生物质分离。由于制浆条件,溶解的木质素解聚,只有75%可以沉淀。为了增加该量,研究了嗜热毁丝霉漆酶与溶解在AlkaPolP工艺黑液中的木质素的24小时反应。使用Box-Behnken因子设计在间歇搅拌釜反应器中检查了pH,温度,酶浓度和氧分压的影响。由于酶促反应,木质素聚合,这导致增强的木质素沉淀。添加介体可改善聚合反应,但减少可沉淀木质素的量。可以使用二阶模型充分描述参数对沉淀产率和分子量的影响,并可以评估最佳条件。所得木质素的FT-IR光谱表明保留了其典型的酚结构。 (C)2015 Elsevier Ltd.保留所有权利。

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