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首页> 外文期刊>Coral reefs: journal of the International Society for Reef Studies >Diel rhythmicity of lipid-body formation in a coral-Symbiodinium endosymbiosis
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Diel rhythmicity of lipid-body formation in a coral-Symbiodinium endosymbiosis

机译：珊瑚共生菌共生中脂质体形成的狄尔节律

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The biogenesis of intracellular lipid bodies (LBs) is dependent upon the symbiotic status between host corals and their intracellular dinoflagellates (genus Symbiodinium), though aside from this observation, little is known about LB behavior and function in this globally important endosymbiosis. The present research aimed to understand how LB formation and density are regulated in the gastrodermal tissue layer of the reef-building coral Euphyllia glabrescens. After tissue fixation and labeling with osmium tetroxide, LB distribution and density were quantified by imaging analysis of serial cryo-sections, and a diel rhythmicity was observed; the onset of solar irradiation at sunrise initiated an increase in LB density and size, which peaked at sunset. Both LB density and size then decreased to basal levels at night. On a seasonal timescale, LB density was found to be significantly positively correlated with seasonal irradiation, with highest densities found in the summer and lowest in the fall. In terms of LB lipid composition, only the concentration of wax esters, and not triglycerides or sterols, exhibited diel variability. This suggests that the metabolism and accumulation of lipids in LBs is at least partially light dependent. Ultrastructural examinations revealed that the LB wax ester concentration correlated with the number of electron-transparent inclusion bodies. Finally, there was a directional redistribution of the LB population across the gastroderm over the diel cycle. Collectively, these data reveal that coral gastrodermal LBs vary in composition and intracellular location over diel cycles, features which may shed light on their function within this coral-dinoflagellate mutualism.
机译：细胞内脂质体（LBs）的生物发生取决于寄主珊瑚与其细胞内鞭毛鞭毛藻（共生双歧杆菌属）之间的共生状态，尽管除此观察外，人们对这种在全球范围内重要的共生体中的LB行为和功能知之甚少。本研究旨在了解如何在造礁珊瑚Euphyllia glabrescens的胃真皮组织层中调节LB的形成和密度。组织固定并用四氧化标记后，通过连续冷冻切片的成像分析定量LB分布和密度，并观察到迪尔节律。日出时太阳辐射的开始导致LB密度和大小的增加，并在日落时达到峰值。夜间，LB的密度和大小均下降至基础水平。在季节尺度上，发现LB密度与季节辐照显着正相关，在夏季发现最高密度，在秋天发现最低密度。就LB脂质组成而言，仅蜡酯而不是甘油三酸酯或固醇的浓度表现出狄尔变异性。这表明LB中脂质的代谢和积累至少部分是光依赖性的。超微结构检查表明，LB蜡酯的浓度与电子透明包涵体的数量有关。最后，在整个diel循环中，LB群体在整个胃胚中有方向性的重新分布。总的来说，这些数据表明，珊瑚胃胚LB在diel周期内的组成和细胞内位置各不相同，这些特征可能揭示了它们在这种珊瑚-鞭毛虫共生中的功能。

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