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首页> 外文期刊>Brain research >Transient up-regulation of cocaine- and amphetamine-regulated transcript peptide (CART) immunoreactivity following ethanol withdrawal in rat hypothalamus.
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Transient up-regulation of cocaine- and amphetamine-regulated transcript peptide (CART) immunoreactivity following ethanol withdrawal in rat hypothalamus.

机译：大鼠下丘脑中乙醇戒断后可卡因和苯丙胺调节的转录肽（CART）免疫反应性的瞬时上调。

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摘要

We investigated the profile of CART immunoreactivity in some discrete hypothalamic nuclei following chronic ethanol treatment and withdrawal conditions. Adult, male, Sprague-Dawley rats were fed with liquid diet (pair-fed) or liquid diet containing ethanol (ethanol-fed) for 15 days. Thereafter, all the animals were given access to ethanol free nutritionally balanced liquid diet and killed at 0, 24, 48 and 72 h post-withdrawal, and their brains processed for immunocytochemistry using monoclonal antibodies against CART. CART-immunoreactive fibers, but not the cells, were significantly increased in the paraventricular nucleus (PVN). However, the profile of CART-immunoreactive cells and/or fibers in the periventricular area (PeA), arcuate nucleus (ARC), perifornical area inclusive of lateral hypothalamus (LH) and tuber cinereum (TC), dorsomedial (DMH), and ventromedial (VMH) hypothalamus at the 0 h ethanol withdrawal time point was quite similar to that in the pair-fed control rats. Twenty-four hours following ethanol withdrawal, the immunoreactivity in all these areas was dramatically increased. While significant reduction in CART immunoreactivity was noticed in the PVN, PeA, ARC and VMH at 48 h, immunoreactive profile was restored to normal by 72 h post-ethanol withdrawal. The immunoreactive profile in the LH, TC and DMH resembled that of the pair-fed groups at 48 and 72 h post-withdrawal intervals. However, CART-immunoreactive profile in the supraoptic nucleus did not respond to the chronic ethanol treatment and/or withdrawal. We suggest that transient up-regulation of CART in some discrete hypothalamic nuclei following ethanol withdrawal, at least in part, may contribute to the pathogenesis of ethanol withdrawal-induced symptoms like anxiety and anorexia.
机译：我们调查了慢性乙醇治疗和戒断条件后一些离散的下丘脑核中CART免疫反应性的概况。成年，雄性，Sprague-Dawley大鼠以液态饮食（配对喂养）或含乙醇的液态饮食（乙醇喂养）喂养15天。此后，使所有动物获得无乙醇营养均衡的流质饮食，并在撤离后0、24、48和72小时处死，并使用针对CART的单克隆抗体对它们的大脑进行免疫细胞化学处理。 CART免疫反应性纤维，但不是细胞，在室旁核（PVN）中显着增加。但是，CART免疫反应性细胞和/或纤维的分布在脑室周围区域（PeA），弓形核（ARC），包括下丘脑外侧（LH）和块茎灰质（TC）在内的肾上腺区，背囊（DMH）和腹膜乙醇撤离时间点0h的下丘脑（VMH）与成对喂养的对照大鼠非常相似。撤除乙醇后二十四小时，所有这些区域的免疫反应性均显着提高。尽管在48 h时PVN，PeA，ARC和VMH中CART免疫反应性显着降低，但在撤离乙醇72小时后，免疫反应性已恢复正常。 LH，TC和DMH中的免疫反应曲线与成对进食组在退出后48和72小时的免疫反应曲线相似。但是，视上核中的CART免疫反应谱对慢性乙醇治疗和/或停药无反应。我们建议，乙醇戒断后某些离散的下丘脑核中CART的瞬时上调至少部分可能与乙醇戒断引起的焦虑和厌食症状有关。

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