...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Brain research >The Kv1.2 potassium channel: the position of an N-glycan on the extracellular linkers affects its protein expression and function.
【24h】

The Kv1.2 potassium channel: the position of an N-glycan on the extracellular linkers affects its protein expression and function.

机译：Kv1.2钾通道：N-聚糖在细胞外接头上的位置会影响其蛋白质表达和功能。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Voltage-gated potassium Kv1 channels have three extracellular linkers, the S1-S2, the S3-S4, and the S5-P. The S1-S2 is the only linker that has an N-glycan and it is at a conserved position on this linker on Kv1.1-Kv1.5 and Kv1.7 channels. We hypothesize that an N-glycan is found at only this position due to its effect on folding, trafficking, and/or function of these channels. To investigate this hypothesis, N-glycosylation sites were engineered at different positions on the extracellular linkers of Kv1.2 to determine the effects of N-glycans on channel surface protein expression and function. Our data suggest that for Kv1 channels, (1) placing an N-glycan at non-native positions on the S1-S2 linker decreased cell surface protein expression but the N-glycan still affected function similarly as if it were at its native position, (2) placing a non-native N-glycan on the S3-S4 linker significantly altered function, and (3) placing a non-native N-glycan on the S5-P linker disrupted both trafficking and function. We suggest that Kv1 channels have an N-glycan at a conserved position on only the S1-S2 linker to overcome the constraints for proper folding, trafficking, and function that appear to occur if the N-glycan is moved from this position.
机译：电压门控钾Kv1通道具有三个细胞外接头，即S1-S2，S3-S4和S5-P。 S1-S2是唯一具有N-聚糖的接头，在Kv1.1-Kv1.5和Kv1.7通道上，该接头位于该接头的保守位置。我们假设由于其对这些通道的折叠，运输和/或功能的影响，仅在该位置发现了N-聚糖。为了研究该假设，在Kv1.2的胞外接头上的不同位置设计了N-糖基化位点，以确定N-聚糖对通道表面蛋白表达和功能的影响。我们的数据表明，对于Kv1通道，（1）将N-聚糖放置在S1-S2接头的非天然位置会降低细胞表面蛋白的表达，但N-聚糖仍会像在其天然位置一样影响其功能， （2）在S3-S4接头上放置非天然N-聚糖会显着改变功能，并且（3）在S5-P接头上放置非天然N-聚糖会破坏运输和功能。我们建议Kv1通道仅在S1-S2接头上的保守位置具有N-聚糖，以克服N-聚糖从该位置移动时出现的正确折叠，运输和功能的限制。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号