...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Brain research >Ginsenosides Rb1 and Rg1 promote proliferation and expression of neurotrophic factors in primary Schwann cell cultures.
【24h】

Ginsenosides Rb1 and Rg1 promote proliferation and expression of neurotrophic factors in primary Schwann cell cultures.

机译：人参皂甙Rb1和Rg1促进了Schwann原代细胞培养物中神经营养因子的增殖和表达。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Ginsenoside Rb1 (GRb1) and ginsenoside Rg1 (GRg1), two major ingredients in ginseng root, have gained extensive attention because of its neuroprotective properties. Thus far, most of the studies on GRb1 and GRg1 have been focused on their neuroprotective effects on neurons. The potential beneficial effects of GRb1 and GRg1 on Schwann cells have not been investigated comprehensively. The present study was designed to examine the possible beneficial effect of GRb1 and GRg1 on proliferation and expression of nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) in Schwann cells. Schwann cells were incubated without or with GRb1 and GRg1 at different doses. The proliferation of Schwann cells was examined by cell counting. The expression and secretion of NGF and BDNF were examined by western blotting and ELISA. We found that both GRb1 and GRg1 were capable of increasing the proliferation of, and the expression and secretion of NGF and BDNF in Schwann cells. Further studies showed that both GRb1 and GRg1 were able to increase intracellular cyclic AMP (cAMP) level and protein kinase A (PKA) activity. Preincubation with 10 muM H89 (a PKA inhibitor) significantly inhibited the beneficial effects of GRb1 and GRg1 on Schwann cells. These findings indicate that the beneficial effects of GRb1 and GRg1 on proliferation and expression of NGF and BDNF occurs mainly through the PKA pathway in cultured Schwann cells.
机译：人参根中的两种主要成分人参皂苷Rb1（GRb1）和人参皂苷Rg1（GRg1）由于其神经保护特性而受到广泛关注。迄今为止，关于GRb1和GRg1的大多数研究都集中在它们对神经元的神经保护作用上。尚未全面研究GRb1和GRg1对雪旺细胞的潜在有益作用。本研究旨在检查GRb1和GRg1对雪旺细胞中神经生长因子（NGF）和脑源性神经营养因子（BDNF）增殖和表达的可能的有益作用。雪旺氏细胞在不使用GRb1和GRg1的情况下或以不同剂量孵育。通过细胞计数检查了雪旺氏细胞的增殖。通过蛋白质印迹和ELISA检测NGF和BDNF的表达和分泌。我们发现GRb1和GRg1都能够增加Schwann细胞中NGF和BDNF的增殖以及其表达和分泌。进一步的研究表明GRb1和GRg1均能够增加细胞内环AMP（cAMP）水平和蛋白激酶A（PKA）活性。与10μMH89（一种PKA抑制剂）进行预温育可显着抑制GRb1和GRg1对雪旺细胞的有益作用。这些发现表明，GRb1和GRg1对NGF和BDNF的增殖和表达的有益作用主要通过培养的施旺细胞中的PKA途径发生。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号