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首页> 外文期刊>Brain research >The different HMGA1 expression of total population of glioblastoma cell line U251 and glioma stem cells isolated from U251
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The different HMGA1 expression of total population of glioblastoma cell line U251 and glioma stem cells isolated from U251

机译：胶质母细胞瘤细胞系U251和分离自U251的神经胶质瘤干细胞总群体的HMGA1表达不同

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The high-mobility group Al (HMGA1) protein is a non-histone architectural nuclear factor and participates in diverse biological processes, including gene transcription, embryogenesis, cell cycle regulation, apoptosis, and even neoplastic transformation. In our study, glioma stem cells (GSCs) expressing the surface marker CD133 from human glioblastoma cell line U251 were isolated using MACS column and were analyzed using immunofluorescence and flow cytometry (FCM). The different expression of HMGA1 was detected using real-time RT-PCR and Western blot at transcriptional and translational levels between U251 and isolated GSCs. The results show that GSCs were successfully isolated from U251 and cultured in serum-free medium (SMF). The percentage of GSCs in U251 was 0.32%?07%. HMGA1 expression was significantly higher in GSCs than in glioblastoma cells (P<0.05), up to 6.13?25-fold and 2.75? 0.99-fold at transcriptional and translational levels, respectively. These results indicated HMGA1 is overexpressed in GSCs as compared to glioblastoma cell line U251, which points to the expression of HMGA1 being closely related to malignant proliferation, invasion, and differentiation of tumors from the prospective of tumor stem cells (TSCs). We conclude that HMGA1 may be a potential biomarker and rational therapeutic target for glioblastoma and GSC.
机译：高迁移率的A1组（HMGA1）蛋白是一种非组蛋白的建筑核因子，参与多种生物过程，包括基因转录，胚胎发生，细胞周期调控，细胞凋亡，甚至是肿瘤转化。在我们的研究中，使用MACS柱分离了表达自人胶质母细胞瘤细胞U251的表面标记CD133的神经胶质瘤干细胞（GSC），并使用免疫荧光和流式细胞术（FCM）进行了分析。使用实时RT-PCR和Western blot在U251和分离的GSC之间的转录和翻译水平检测到HMGA1的不同表达。结果表明，成功地从U251中分离出GSC，并在无血清培养基（SMF）中进行培养。 U251中GSC的百分比为0.32％？07％。 GSCs中HMGA1的表达明显高于胶质母细胞瘤细胞（P <0.05），分别高达6.13？25倍和2.75?。在转录和翻译水平上分别为0.99倍。这些结果表明，与胶质母细胞瘤细胞系U251相比，HMGA1在GSC中过表达，这表明HMGA1的表达与预期的肿瘤干细胞（TSC）的恶性增殖，侵袭和分化密切相关。我们得出结论，HMGA1可能是胶质母细胞瘤和GSC的潜在生物标志物和合理的治疗靶标。

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