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首页> 外文期刊>Brain research >Up-regulated ephrinB3/EphB3 expression in intractable temporal lobe epilepsy patients and pilocarpine induced experimental epilepsy rat model
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Up-regulated ephrinB3/EphB3 expression in intractable temporal lobe epilepsy patients and pilocarpine induced experimental epilepsy rat model

机译：难治性颞叶癫痫患者和毛果芸香碱诱导的实验性癫痫大鼠模型中ephrinB3 / EphB3表达上调

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EphB family receptor tyrosine kinases, in cooperation with cell surface-bound ephrinB ligands, play a critical role in maintenance of dendritic spine morphogenesis, axons guidance, synaptogenesis, synaptic reorganization and plasticity in the central nervous system (CNS). However, the expression pattern of ephrinB/EphB in intractable temporal lobe epilepsy (TLE) and the underlying molecular mechanisms during epileptogenesis remain poorly understood. Here we investigated the expression pattern and cellular distribution of ephrinB/EphB in intractable TLE patients and lithium chloride-pilocarpine induced TLE rats using real-time quantitative polymerase chain reaction (RT-qPCR), immunohistochemistry, double-labeled immunofluorescence and Western blot analysis. Compared to control groups, ephrinB3 and EphB3 mRNA expression were significantly up regulated in intractable TLE patients and TLE rats, while the mRNA expression trend of ephrinB1/2 and EphB1/2/4/6 in intractable TLE patients and TLE rats were inconsistent. Western blot analysis and semi-quantitative immunohistochemistry confirmed that ephrinB3 and EphB3 protein level were up-regulated in intractable TLE patients and TLE rats. At the same time, double-labeled immunofluorescence indicate that ephrinB3 was expressed mainly in the cytoplasm and protrusions of glia and neurons, while EphB3 was expressed mainly in the cytoplasm of neurons. Taken together, up-regulated expression of ephrinB3/EphB3 in intractable TLE patients and experimental TLE rats suggested that ephrinB3/EphB3 might be involved in the pathogenesis of TLE. (C) 2016 Elsevier B.V. All rights reserved.
机译：EphB家族受体酪氨酸激酶与细胞表面结合的ephrinB配体合作，在维持树突棘形态，轴突引导，突触形成，突触重组和中枢神经系统（CNS）的维持中起关键作用。然而，ephrinB / EphB在顽固性颞叶癫痫（TLE）中的表达模式和癫痫发生过程中的潜在分子机制仍然知之甚少。在这里，我们使用实时定量聚合酶链反应（RT-qPCR），免疫组织化学，双标记免疫荧光和蛋白质印迹分析研究了难治性TLE患者和氯化锂-毛果芸香碱诱导的TLE大鼠中ephrinB / EphB的表达模式和细胞分布。与对照组相比，难治性TLE患者和TLE大鼠的ephrinB3和EphB3 mRNA表达明显上调，而难治性TLE患者和TLE大鼠中ephrinB1 / 2和EphB1 / 2/4/6的mRNA表达趋势不一致。蛋白质印迹分析和半定量免疫组织化学证实，顽固性TLE患者和TLE大鼠中ephrinB3和EphB3蛋白水平上调。同时，双标记免疫荧光表明ephrinB3主要在神经胶质和神经元的细胞质和突起中表达，而EphB3主要在神经元的细胞质中表达。总之，难治性TLE患者和实验性TLE大鼠中ephrinB3 / EphB3的表达上调提示ephrinB3 / EphB3可能与TLE的发病有关。 （C）2016 Elsevier B.V.保留所有权利。

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