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首页> 外文期刊>Chemistry: A European journal >Magnetic electrochemiluminescent Fe3O4/CdSe-CdS nanoparticle/polyelectrolyte nanocomposite for highly efficient immunosensing of a cancer biomarker.
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Magnetic electrochemiluminescent Fe3O4/CdSe-CdS nanoparticle/polyelectrolyte nanocomposite for highly efficient immunosensing of a cancer biomarker.

机译：磁性电化学发光Fe3O4 / CdSe-CdS纳米颗粒/聚电解质纳米复合材料，用于癌症生物标记物的高效免疫感应。

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Magnetic electrochemiluminescent Fe(3)O(4)/CdSe-CdS nanoparticle/polyelectrolyte nanostructures have been synthesized and used to fabricate an electrochemiluminescence (ECL) immunosensor for the detection of carcinoembryonic antigen (CEA). CEA is a protein used as a biomarker for several cancers; particularly, to monitor response to treatment in colon and rectal cancer patients. The nanocomposites can be easily separated and firmly attached to an electrode owing to their excellent magnetic properties. This represents a promising advantage for bioassay applications. More importantly, the nanostructures exhibit intense and stable ECL emissions in neutral solution, which makes them ideal for ECL immunosensing. The 3-aminopropyltriethoxysilane (APS) polyelectrolyte shell on the nanostructure surface not only enhances the intensity and stability of the ECL signal, but also acts as a crosslinker for immunosensor fabrication. A CEA antibody immobilized onto a nanocomposite/APS/electrode with gold nanoparticles comprises the ECL immunosensor. The principle of ECL detection for CEA is based on a change in steric hindrance after immunoreaction, which leads to a decrease in ECL intensity. A wide detection range (0.064pg ml(-1)-10ng ml(-1)) and low detection limit (0.032pg ml(-1)) are achieved. The immunosensor is highly sensitive and selective, and exhibits excellent stability and good reproducibility. It thus has great potential for clinical protein detection. In particular, this approach uses a novel class of bifunctional nanocomposites that display both intense ECL and excellent magnetism, which renders them suitable for a large range of bioassay applications.
机译：磁性电化学发光Fe（3）O（4）/ CdSe-CdS纳米颗粒/聚电解质纳米结构已被合成，并用于制造用于检测癌胚抗原（CEA）的电致发光（ECL）免疫传感器。 CEA是一种蛋白质，可用于多种癌症的生物标记。特别是监测结肠癌和直肠癌患者对治疗的反应。纳米复合材料具有出色的磁性，因此可以轻松分离并牢固地附着在电极上。这代表了生物测定应用的有前途的优势。更重要的是，纳米结构在中性溶液中表现出强烈且稳定的ECL发射，这使其成为ECL免疫传感的理想选择。纳米结构表面上的3-氨丙基三乙氧基硅烷（APS）聚电解质壳不仅增强了ECL信号的强度和稳定性，而且还充当免疫传感器制造的交联剂。用金纳米颗粒固定在纳米复合材料/ APS /电极上的CEA抗体包含ECL免疫传感器。 CEA的ECL检测原理基于免疫反应后的空间位阻变化，从而导致ECL强度降低。实现了较宽的检测范围（0.064pg ml（-1）-10ng ml（-1））和较低的检测限（0.032pg ml（-1））。该免疫传感器是高度敏感和选择性的，并且表现出优异的稳定性和良好的再现性。因此，它具有临床蛋白质检测的巨大潜力。特别地，该方法使用一类新型的双功能纳米复合材料，该复合材料既显示出强烈的ECL，又具有出色的磁性，这使其适用于多种生物测定应用。

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