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首页> 外文期刊>Chemistry: A European journal >Label-free, non-derivatization CRET detection platform for 6-mercaptopurine based on the distance-dependent optical properties of gold nanoparticles
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Label-free, non-derivatization CRET detection platform for 6-mercaptopurine based on the distance-dependent optical properties of gold nanoparticles

机译：基于金纳米粒子的距离依赖的光学特性，用于6-巯基嘌呤的无标记，非衍生化CRET检测平台

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摘要

A label-free, non-derivatization chemiluminescence resonance energy transfer (CRET) detection platform has been developed for the detection of the non-fluorescent small molecule 6-mercaptopurine. This CRET process arose from a chemiluminescent (CL) donor-acceptor system in which the reaction of bis(2,4,6-trichlorophenyl)oxalate (TCPO)-H _2O _2-fluorescein (maximum emission at 521.6 nm) served as the donor and gold nanoparticles (AuNPs, maximum absorption at 520.0 nm) served as the acceptor. This process caused a significant decrease in the CL signal of the TCPO-H _2O _2-fluorescein reaction. The presence of 6-mercaptopurine induced an aggregation of AuNPs with the assistance of Cu ~(2+) ions through cooperative metal-ligand interactions that was accompanied by a distinct change in color and optical properties. The maximum absorption band of the AuNPs was red-shifted to 721.0 nm and no longer overlapped with the CL spectrum of the reaction; as a result, the CL signal was restored. This CRET system exhibited a wide linear range, from 9.0 nmol L ~(-1) to 18.0 μmol L ~(-1), and a low detection limit (0.62 nmol L ~(-1)) for 6-mercaptopurine. The applicability of the proposed CRET system was evaluated by analysis of 6-mercaptopurine in spiked human plasma samples. A label-free, non-derivatization CRET platform was designed by using distance-dependent optical properties of Au nanoparticles (see figure). This platform offers a simple and sensitive method for the detection of substances that modulate the color of Au nanoparticles.
机译：已经开发了一种无标记，非衍生化化学发光共振能量转移（CRET）检测平台，用于检测非荧光小分子6-巯基嘌呤。此CRET过程源自化学发光（CL）供体-受体系统，其中双（2,4,6-三氯苯基）草酸酯（TCPO）-H _2O _2-荧光素（最大发射在521.6 nm）的反应用作供体金纳米颗粒（AuNPs，在520.0 nm处有最大吸收）用作受体。此过程导致TCPO-H _2O _2-荧光素反应的CL信号大大降低。 6-巯基嘌呤的存在通过协同的金属-配体相互作用在Cu〜（2+）离子的辅助下诱导了AuNP的聚集，伴随着颜色和光学性质的明显变化。 AuNPs的最大吸收带红移至721.0 nm，不再与反应的CL光谱重叠;结果，CL信号被恢复。该CRET系统显示出从9.0 nmol L〜（-1）到18.0μmolL〜（-1）的宽线性范围，并且对6-巯基嘌呤的检测限较低（0.62 nmol L〜（-1））。通过分析加标的人血浆样品中的6-巯基嘌呤评估了拟议中的CRET系统的适用性。通过使用与金纳米颗粒的距离相关的光学特性，设计了无标签，非衍生化的CRET平台（见图）。该平台为检测调节金纳米颗粒颜色的物质提供了一种简单而灵敏的方法。

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