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首页> 外文期刊>Chemistry: A European journal >Novel application of Ag nanoclusters in fluorescent imaging of human serum proteins after native polyacrylamide gel electrophoresis (PAGE)
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Novel application of Ag nanoclusters in fluorescent imaging of human serum proteins after native polyacrylamide gel electrophoresis (PAGE)

机译：Ag纳米簇在天然聚丙烯酰胺凝胶电泳（PAGE）后在人血清蛋白荧光成像中的新应用

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摘要

We have developed a novel application for DNA oligonucleotidestabilized Ag nanoclusters in fluorescent imaging of human serum proteins after native polyacrylamide gel electrophoresis (PAGE). Oligonucleotide-stabilized Ag nanoclusters were used as fluorescent probes for direct detection of proteins after native PAGE. Some relatively low-abundance proteins, such as a-1-antichymotrypsin (ACT) and a- 2-glycoprotein 1, zinc (ZAG) were easily detected by oligonucleotide-stabilized Ag nanocluster-based fluorescent imaging and identified by MS and MS/MS techniques, without the need of expensive antibodies or tedious immunoassay procedures. The pH condition for the oligonucleotide-stabilized Ag nanocluster solution was optimized and the possible mechanism of interaction between proteins and DNA oligonucleotide- stabilized Ag nanoclusters was analyzed. As a novel fluorescent detection method it is simple, fast, nontoxic and sensitive, and it shows great analytical potential in proteome research and in biochemistry.
机译：在天然聚丙烯酰胺凝胶电泳（PAGE）后，我们开发了一种DNA寡核苷酸稳定的Ag纳米簇在人类血清蛋白的荧光成像中的新应用。寡核苷酸稳定的Ag纳米簇被用作荧光探针，用于天然PAGE后直接检测蛋白质。通过寡核苷酸稳定的基于Ag纳米簇的荧光成像很容易检测到一些相对低丰度的蛋白质，例如a-1-抗胰凝乳蛋白酶（ACT）和a-2-糖蛋白1，锌（ZAG），并通过MS和MS / MS进行鉴定技术，而无需昂贵的抗体或繁琐的免疫测定程序。优化了寡核苷酸稳定的Ag纳米簇溶液的pH条件，并分析了蛋白质与DNA寡核苷酸稳定的Ag纳米簇之间相互作用的可能机理。作为一种新颖的荧光检测方法，它简单，快速，无毒且灵敏，并且在蛋白质组学研究和生物化学中显示出巨大的分析潜力。

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