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首页> 外文期刊>Chemistry: A European journal >Synthesis of thermo- and pH-sensitive polyion complex micelles for fluorescent imaging
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Synthesis of thermo- and pH-sensitive polyion complex micelles for fluorescent imaging

机译：合成热敏和pH敏感的聚离子复合胶束，用于荧光成像

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Two thermo- and pH-sensitive polypeptide-based copolymers, poly(N-isopropylacrylamide-co-N-hydroxymethylacrylamide)-b-poly(L-lysine) (P(NIPAAm-co-HMAAm)-b-PLL, P1) and poly(N-isopropylacrylamide-co-N- hydroxymethylacrylamide)-b-poly(glutamic acid) (P(NIPAAm-co-HMAAm)-b-PGA, P2), have been designed and synthesized by the ring-opening anionic polymerization of N-carboxyanhydrides (NCA) with amino-terminated P(NIPAAm-co-HMAAm). It was found that the block copolymers exhibit good biocompatibility and low toxicity. As a result of electrostatic interactions between the positively charged PLL and negatively charged PGA, P1 and P2 formed polyion complex (PIC) micelles consisting of polyelectrolyte complex cores and P(NIPAAm-co-HMAAm) shells in aqueous solution. The thermo- and pH-sensitivity of the PIC micelles were studied by UV/Vis spectrophotometry, dynamic light scattering (DLS), and transmission electron microscopy (TEM). Moreover, fluorescent PIC micelles were achieved by introducing two fluorescent molecules with different colors. Photographs and confocal laser scanning microscopy (CLSM) showed that the fluorescence-labeled PIC micelles exhibit thermo- and pH-dependent fluorescence, which may find wide applications in bioimaging in complicated microenvironments.
机译：两种基于热和pH敏感的基于多肽的共聚物，聚（N-异丙基丙烯酰胺-共-N-羟甲基丙烯酰胺）-b-聚（L-赖氨酸）（P（NIPAAm-co-HMAAm）-b-PLL，P1）和聚（N-异丙基丙烯酰胺-共-N-羟甲基丙烯酰胺）-b-聚谷氨酸（P（NIPAAm-共-HMAAm）-b-PGA，P2）已通过开环阴离子聚合法设计和合成N-羧基酐（NCA）与氨基末端的P（NIPAAm-co-HMAAm）。发现该嵌段共聚物表现出良好的生物相容性和低毒性。由于带正电的PLL与带负电的PGA之间的静电相互作用，P1和P2在水溶液中形成了由聚电解质复合物核和P（NIPAAm-co-HMAAm）壳组成的聚离子复合物（PIC）胶束。通过紫外/可见光分光光度法，动态光散射（DLS）和透射电子显微镜（TEM）研究了PIC胶束的热敏感性和pH敏感性。此外，通过引入两个具有不同颜色的荧光分子来实现荧光PIC胶束。照片和共聚焦激光扫描显微镜（CLSM）表明，荧光标记的PIC胶束具有依赖于温度和pH的荧光，在复杂的微环境中的生物成像中可能会找到广泛的应用。

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