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首页> 外文期刊>Chemistry: A European journal >Structural changes in a protein fragment from abalone shell during the precipitation of calcium carbonate
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Structural changes in a protein fragment from abalone shell during the precipitation of calcium carbonate

机译：碳酸钙沉淀过程中鲍鱼壳蛋白质片段的结构变化

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摘要

Mineralized tissues grow through biologically controlled processes in which specific macromolecules are involved. Some of these molecules, which are present in very low concentrations and are difficult to localize and characterize, become entrapped inside the mineralized tissue. Herein, a protein fragment, GP, which was obtained by the alkaline digestion of the green sheet of the abalone shell, is used as a probe to study the changes in molecular structure that occur during the precipitation of calcium carbonate. This important goal was achieved by exploiting a fluorescent tag in GP. The experimental results that were obtained by using spectroscopic-, chromatographic-, and microscopic techniques indicate that GP controls the precipitation kinetics and morphology of calcium carbonate crystals, and that it only undergoes structural reorganization when entrapped inside calcium carbonate crystals. To the best of our knowledge, this report represents one of the first studies on the conformational changes of a protein fragment that is involved in biomineralization processes on moving from the solution phase into the mineral phase. Coming out of your shell: A natural protein fragment in the shells of abalone (see figure), which has fluorescent properties, is used to probe the conformational changes that occur when it moves from solution into a calcite crystal. This fragment is mainly unfolded in solution and only changes its conformation when entrapped inside the crystal.
机译：矿化的组织通过涉及特定大分子的生物控制过程生长。这些分子中的某些以非常低的浓度存在并且难以定位和表征，因此被困在矿化组织内部。在此，通过碱性消化鲍鱼壳的生片而获得的蛋白质片段GP被用作探针，以研究在碳酸钙沉淀期间发生的分子结构的变化。通过利用GP中的荧光标签可以实现这一重要目标。通过使用光谱，色谱和显微镜技术获得的实验结果表明，GP控制了碳酸钙晶体的沉淀动力学和形态，并且仅当捕获在碳酸钙晶体内部时，GP才会进行结构重组。据我们所知，本报告是有关蛋白质片段构象变化的最早研究之一，该蛋白片段从溶液相进入矿物质相涉及生物矿化过程。脱壳：鲍鱼壳中的天然蛋白质片段（见图）具有荧光特性，可用来探测从溶液进入方解石晶体时发生的构象变化。该片段主要在溶液中展开，并且仅当截留在晶体内部时才改变其构象。

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