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首页> 外文期刊>Chemistry: A European journal >Solution structure and conformational dynamics of deoxyxylonucleic acids (dXNA): An orthogonal nucleic acid candidate
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Solution structure and conformational dynamics of deoxyxylonucleic acids (dXNA): An orthogonal nucleic acid candidate

机译：脱氧木糖核酸（dXNA）的溶液结构和构象动力学：正交核酸候选物

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摘要

Orthogonal nucleic acids are chemically modified nucleic acid polymers that are unable to transfer information with natural nucleic acids and thus can be used in synthetic biology to store and transfer genetic information independently. Recently, it was proposed that xylose-DNA (dXNA) can be considered to be a potential candidate for an orthogonal system. Herein, we present the structure in solution and conformational analysis of two self-complementary, fully modified dXNA oligonucleotides, as determined by CD and NMR spectroscopy. These studies are the initial experimental proof of the structural orthogonality of dXNAs. In aqueous solution, dXNA duplexes predominantly form a linear ladderlike (type-1) structure. This is the first example of a furanose nucleic acid that adopts a ladderlike structure. In the presence of salt, an equilibrium exists between two types of duplex form. The corresponding nucleoside triphosphates (dXNTPs) were synthesized and evaluated for their ability to be incorporated into a growing DNA chain by using several natural and mutant DNA polymerases. Despite the structural orthogonality of dXNA, DNA polymerase β mutant is able to incorporate the dXNTPs, showing DNA-dependent dXNA polymerase activity. The acid test: The structural and biochemical aspects of xylose-DNA (dXNA; see scheme) have been explored, with the aim of using it as an orthogonal nucleic acid system to transfer genetic information without interfering with the natural system. The study identifies the properties for developing dXNA as an alternate information system, although it also finds the reasons why dXNA was not selected as the natural genetic system.
机译：正交核酸是化学修饰的核酸聚合物，不能与天然核酸一起传递信息，因此可以用于合成生物学中以独立存储和传递遗传信息。近来，有人提出木糖-DNA（dXNA）可以被认为是正交系统的潜在候选者。在本文中，我们介绍了CD和NMR光谱法确定的两种自我互补，完全修饰的dXNA寡核苷酸在溶液和构象分析中的结构。这些研究是dXNAs结构正交性的初步实验证明。在水溶液中，dXNA双链体主要形成线性梯状（1型）结构。这是采用梯状结构的呋喃糖核酸的第一个例子。在盐的存在下，两种双链体形式之间存在平衡。合成了相应的三磷酸核苷（dXNTPs），并通过使用几种天然和突变型DNA聚合酶评估了它们被掺入到正在生长的DNA链中的能力。尽管dXNA在结构上是正交的，但DNA聚合酶β突变体仍能够掺入dXNTP，显示出DNA依赖性dXNA聚合酶活性。酸性测试：已经探索了木糖DNA（dXNA;参见方案）的结构和生化方面，目的是将其用作正交核酸系统以在不干扰自然系统的情况下传递遗传信息。该研究确定了将dXNA开发为替代信息系统的特性，尽管它也找到了未选择dXNA作为自然遗传系统的原因。

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