Bi-Enzyme Sensor for Phenolic Compounds with Fluorescent Read-Out

Maria Strianese; Gerhild Zauner; Leandro C Tabares; Armand W. J. W. Tepper; Franco De Martino; Claudio Pellecchia; Thijs J. Aartsma; Gerard W. Canters

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Bi-Enzyme Sensor for Phenolic Compounds with Fluorescent Read-Out

机译：具有荧光读数的酚类化合物双酶传感器

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In this paper, the use of tyrosinase (Ty) from Streptomyces antibioticus, labeled with a fluorescent tag, in combination with soluble quinoprotein (PQQ-containing) glucose dehydrogenase (s-GDH) to measure trace amounts of phenols is explored. Proof of concept is provided by a series of experiments, which show a clear quantitative dependence of the response on the phenol concentration. One of the advantages of the detection system is that apart from a standard fluorimeter no further instrumentation is required.

机译：在本文中，探索了使用带有荧光标记的抗生素链霉菌中的酪氨酸酶（Ty）与可溶性喹蛋白（含PQQ）葡萄糖脱氢酶（s-GDH）结合使用来测定痕量酚的方法。通过一系列实验提供了概念验证，这些实验显示了响应对苯酚浓度的明确定量依赖性。该检测系统的优点之一是，除了标准荧光计以外，不需要其他仪器。

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来源
《Chemistry: A European journal》 |2013年第44期|共6页
作者
Maria Strianese; Gerhild Zauner; Leandro C Tabares; Armand W. J. W. Tepper; Franco De Martino; Claudio Pellecchia; Thijs J. Aartsma; Gerard W. Canters;
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正文语种 eng
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关键词
biosensors; enzymes; FRET; metalloproteins;

机译：生物传感器;酶;FRET;金属蛋白;

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1. Bi-Enzyme Sensor for Phenolic Compounds with Fluorescent Read-Out [J] . Maria Strianese, Gerhild Zauner, Leandro C Tabares, Chemistry: A European journal . 2013,第44期

机译：具有荧光读数的酚类化合物双酶传感器
2. Disposable tyrosinase-peroxidase bi-enzyme sensor for amperometric detection of phenols [J] . Chang SC., Rawson K., McNeil CJ. Biosensors & Bioelectronics: The International Journal for the Professional Involved with Research, Technology and Applications of Biosensers and Related Devices . 2002,第11a12期

机译：一次性酪氨酸酶-过氧化物酶双酶传感器用于苯酚的安培检测
3. Turn-on fluorescent sensor for the detection of glucose using manganese dioxide?phenol formaldehyde resin nanocomposite [J] . Zhong Feng Gao, Asmerom Yohannes Ogbe, Ei Ei Sann, Talanta: The International Journal of Pure and Applied Analytical Chemistry . 2018,第期

机译：用于使用二氧化锰检测葡萄糖的导通荧光传感器？酚醛树脂纳米复合材料
4. Versatile on-chip read-out for compound-eye image sensor array [C] . Boussaid, F., Chen Shoushun, . 2005

机译：复眼图像传感器阵列的多功能片上读数
5. Characterization of diamond sensors in ATLAS pixel read-out electronics. [D] . Robichaud, Adam. 2011

机译：ATLAS像素读出电子设备中金刚石传感器的特性。
6. Use of image analysis to estimate anthocyanin and UV-excited fluorescent phenolic compound levels in strawberry fruit [O] . Yosuke Yoshioka, Masayoshi Nakayama, Yuji Noguchi, 2013

机译：利用图像分析估算草莓果实中花色苷和紫外线激发的荧光酚类化合物的水平
7. Quinoline-tagged fluorescent organic probes for sensing of nitro-phenolic compounds and Zn2+ ions at the ppb level [O] . Gouri Chakraborty, Prasenjit Das, Sanjay K. Mandal 2021

机译：喹啉标记的荧光有机探针，用于检测PPB水平的硝基酚类化合物和Zn2 +离子

Bi-Enzyme Sensor for Phenolic Compounds with Fluorescent Read-Out

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