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首页> 外文期刊>Chemistry: A European journal >Forming lipid bilayer membrane arrays on micropatterned polyelectrolyte film surfaces
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Forming lipid bilayer membrane arrays on micropatterned polyelectrolyte film surfaces

机译：在微图案化的聚电解质膜表面上形成脂质双层膜阵列

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A novel method of forming lipid bilayer membrane arrays on micropatterned polyelectrolyte film surfaces is introduced. Polyelectrolyte films were fabricated by the layer-by-layer technique on a silicon oxide surface modified with a 3-aminopropyltriethoxysilane (APTES) monolayer. The surface pK _a value of the APTES monolayer was determined by cyclic voltammetry to be approximately 5.61, on the basis of which a pH value of 2.0 was chosen for layer-by-layer assembly. Micropatterned polyelectrolyte films were obtained by deep-UV (254 nm) photolysis though a mask. Absorbed fluorescent latex beads were used to visualize the patterned surfaces. Lipid bilayer arrays were fabricated on the micropatterned surfaces by immersing the patterned substrates into a solution containing egg phosphatidylcholine vesicles. Fluorescence recovery after photobleaching studies yielded a lateral diffusion coefficient for probe molecules of 1.31±0.17 μm~2 s~(-1) in the bilayer region, and migration of the lipid NBD PE in bilayer lipid membrane arrays was observed in an electric field. Layer-by-layer assembly of polyelectrolyte films on modified glass substrates, followed by micropatterning by deep-UV photolysis though a mask and immersion of the patterned substrates in a solution of lipid vesicles, afforded lipid bilayer arrays (see figure). The migration behavior of charged lipids in the lipid bilayer arrays under an electric field suggests that these patterned supported bilayers may have applications in studies on the manipulation of charged species in the membrane.
机译：介绍了一种在微图案化聚电解质薄膜表面形成脂质双层膜阵列的新方法。通过逐层技术在经3-氨丙基三乙氧基硅烷（APTES）单层改性的氧化硅表面上制备聚电解质薄膜。通过循环伏安法测定APTES单层的表面pK _a值约为5.61，在此基础上选择pH值为2.0进行逐层组装。通过深紫外（254nm）光解通过掩模获得微图案化的聚电解质膜。吸收的荧光胶乳珠用于可视化图案化的表面。通过将图案化的基质浸入含有卵磷脂酰胆碱囊泡的溶液中，在微图案化的表面上制备脂质双层阵列。光漂白研究后的荧光恢复产生了在双层区域中探针分子的横向扩散系数为1.31±0.17μm〜2 s〜（-1），并且在电场中观察到脂质NBD PE在双层脂质膜阵列中的迁移。在修饰的玻璃基板上逐层组装聚电解质薄膜，然后通过深紫外光分解（通过掩模）进行微图案化，然后将图案化的基板浸入脂质囊泡溶液中，从而制得脂质双层阵列（见图）。带电脂质在脂质双层阵列中在电场下的迁移行为表明，这些图案化的支持双层可能在膜中带电物质的操纵研究中具有应用。

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