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首页> 外文期刊>Chemistry: A European journal >DNA Aptamers are Functional Molecular Recognition Sensors in Protic Ionic Liquids
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DNA Aptamers are Functional Molecular Recognition Sensors in Protic Ionic Liquids

机译：DNA适体是质子离子液体中的功能性分子识别传感器

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摘要

The function and structural changes of an AMP molecular aptamer beacon and its molecular recognition capacity for its target, adenosine monophosphate (AMP), was systematically explored in solution with a protic ionic liquid, ethylammonium nitrate (EAN). It could be proven that up to 2m of EAN in TBS buffer, the AMP molecular aptamer beacon was still capable of recognizing AMP while also maintaining its specificity. The specificity was proven by using the guanosine monophosphate (GMP) as target; GMP is structurally similar to AMP but was not recognized by the aptamer. We also found that in highly concentrated EAN solutions the overall amount of double stranded DNA formed, as well as its respective thermal stability, diminished gradually, but surprisingly the hybridization rate (k_h) of single stranded DNA was significantly accelerated in the presence of EAN. The latter may have important implications in DNA technology for the design of biosensing and DNA-based nanodevices in nonconventional solvents, such as ionic liquids.
机译：在质子离子液体硝酸乙铵（EAN）溶液中系统地研究了AMP分子适体信标的功能和结构变化及其对靶标单磷酸腺苷（AMP）的分子识别能力。可以证明，在TBS缓冲液中长达2m的EAN，AMP分子适体信标仍然能够识别AMP，同时还保持其特异性。以鸟嘌呤单磷酸鸟苷（GMP）为靶标证明了特异性。 GMP在结构上与AMP类似，但适体无法识别。我们还发现，在高度浓缩的EAN溶液中，形成的双链DNA的总量及其各自的热稳定性逐渐降低，但是令人惊讶的是，在EAN的存在下，单链DNA的杂交速率（k_h）显着提高。后者可能对DNA技术中非常规溶剂（如离子液体）中生物传感和基于DNA的纳米器件的设计产生重要影响。

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