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首页> 外文期刊>Chemistry: A European journal >Cyclometalated Iridium(III) Complexes as Two-Photon Phosphorescent Probes for Specific Mitochondrial Dynamics Tracking in Living Cells
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Cyclometalated Iridium(III) Complexes as Two-Photon Phosphorescent Probes for Specific Mitochondrial Dynamics Tracking in Living Cells

机译：环金属化铱（III）配合物作为双光子磷光探针的活细胞中特定线粒体动力学跟踪。

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摘要

Five cyclometalated iridium(III) complexes with 2-phenylimidazo[4,5-f][1,10]phenanthroline derivatives (IrL1-IrL5) were synthesized and developed to image and track mitochondria in living cells under two-photon (750nm) excitation, with two-photon absorption cross-sections of 48.8-65.5GM at 750nm. Confocal microscopy and inductive coupled plasma-mass spectrometry (ICP-MS) demonstrated that these complexes selectively accumulate in mitochondria within 5min, without needing additional reagents for membrane permeabilization, or replacement of the culture medium. In addition, photobleaching experiments and luminescence measurements confirmed the photostability of these complexes under continuous laser irradiation and physiological pH resistance. Moreover, results using 3D multicellular spheroids demonstrate the proficiency of these two-photon luminescent complexes in deep penetration imaging. Two-photon excitation using such novel complexes of iridium(III) for exclusive visualization of mitochondria in living cells may substantially enhance practical applications of bioimaging and tracking.
机译：合成了五种与2-苯基咪唑并[4,5-f] [1,10]菲咯啉衍生物（IrL1-IrL5）的环金属化铱（III）配合物，并在双光子（750nm）激发下显影并跟踪了活细胞中的线粒体。 ，在750nm处具有48.8-65.5GM的双光子吸收截面。共聚焦显微镜和电感耦合等离子体质谱法（ICP-MS）表明，这些络合物在5分钟内选择性积聚在线粒体中，而无需使用其他试剂进行膜透化或更换培养基。另外，光漂白实验和发光测量结果证实了这些络合物在连续激光照射和生理pH抗性下的光稳定性。此外，使用3D多细胞球体的结果证明了这些双光子发光复合体在深度穿透成像中的熟练程度。使用此类新颖的铱（III）络合物进行双光子激发，以实现活细胞中线粒体的唯一可视化，可以大大增强生物成像和跟踪的实际应用。

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