• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Chemistry: A European journal >Fabrication of Highly Stable Glyco-Gold Nanoparticles and Development of a Glyco-Gold Nanoparticle-Based Oriented Immobilized Antibody Microarray for Lectin (GOAL) Assay
【24h】

Fabrication of Highly Stable Glyco-Gold Nanoparticles and Development of a Glyco-Gold Nanoparticle-Based Oriented Immobilized Antibody Microarray for Lectin (GOAL) Assay

机译：高度稳定的糖金纳米颗粒的制备和基于糖金纳米颗粒的凝集素（GOAL）固定化抗体微阵列的开发。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The design of high-affinity lectin ligands is critical for enhancing the inherently weak binding affinities of monomeric carbohydrates to their binding proteins. Glyco-gold nanoparticles (glyco-AuNPs) are promising multivalent glycan displays that can confer significantly improved functional affinity of glyco-AuNPs to proteins. Here, AuNPs are functionalized with several different carbohydrates to profile lectin affinities. We demonstrate that AuNPs functionalized with mixed thiolated ligands comprising glycan (70 mol%) and an amphiphilic linker (30 mol%) provide long-term stability in solutions containing high concentrations of salts and proteins, with no evidence of nonspecific protein adsorption. These highly stable glyco-AuNPs enable the detection of model plant lectins such as Concanavalin A, wheat germ agglutinin, and Ricinus communis Agglutinin 120, at subnanomolar and low picomolar levels through UV/Vis spectrophotometry and dynamic light scattering, respectively. Moreover, we develop in situ glyco-AuNPs-based agglutination on an oriented immobilized antibody microarray, which permits highly sensitive lectin sensing with the naked eye. In addition, this microarray is capable of detecting lectins presented individually, in other environmental settings, or in a mixture of samples. These results indicate that glyconanoparticles represent a versatile and highly sensitive method for detecting and probing the binding of glycan to proteins, with significant implications for the construction of a variety of platforms for the development of glyconanoparticle-based biosensors.
机译：高亲和力凝集素配体的设计对于增强单体碳水化合物与其结合蛋白固有的弱结合亲和力至关重要。糖金纳米颗粒（glyco-AuNPs）是有前途的多价聚糖展示，可显着提高糖AuNPs对蛋白质的功能亲和力。在这里，AuNP被几种不同的碳水化合物官能化，以分析凝集素的亲和力。我们证明AuNPs功能化与混合硫醇化的配体，包括聚糖（70摩尔％）和两亲性连接体（30摩尔％）提供长期稳定的解决方案中包含高浓度的盐和蛋白质，没有非特异性蛋白质吸附的证据。这些高度稳定的糖AuNP可通过UV / Vis分光光度法和动态光散射分别检测亚纳摩尔和低皮摩尔水平的模型植物凝集素，例如伴刀豆球蛋白A，小麦胚芽凝集素和蓖麻蓖麻凝集素120。此外，我们在定向的固定抗体微阵列上开发了基于糖-AuNPs的原位凝集，该凝集可以用肉眼对高凝集素进行传感。另外，该微阵列能够检测在其他环境条件下或样品混合物中单独存在的凝集素。这些结果表明，糖纳米颗粒代表了一种通用且高度灵敏的方法，用于检测和探测聚糖与蛋白质的结合，对构建基于糖纳米颗粒的生物传感器的各种平台的构建具有重要意义。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号