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首页> 外文期刊>Chemistry: A European journal >Labeling and Selective Inactivation of Gram-Positive Bacteria Employing Bimodal Photoprobes with Dual Readouts
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Labeling and Selective Inactivation of Gram-Positive Bacteria Employing Bimodal Photoprobes with Dual Readouts

机译：使用双读数双峰光电探针对革兰氏阳性细菌进行标记和选择性灭活

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摘要

Carbohydrate-conjugated silicon(IV) phthalocyanines with bimodal photoactivity were developed as probes with both fluorescent labeling and photosensitizing capabilities, and the concomitant fluorescent labeling and photoinduced inactivation of Gram-positive and Gram-negative models was explored. The maltohexaose-conjugated photoprobe provides a dual readout to distinguish between both groups of pathogens, as only the Gram-positive species was inactivated, even though both appeared labeled with near-infrared luminescence. Antibiotic resistance did not hinder the phototoxic effect, as even the methicillin-resistant pathogen Staphylococcus aureus (MRSA) was completely photoinactivated. Time-resolved confocal fluorescence microscopy analysis suggests that the photoprobe sticks onto the outer rim of the microorganisms, explaining the resistance of Gram-negative species on the basis of their membrane constitution. The mannose-conjugated photoprobe yields a different readout because it is able to label and to inactivate only the Gram-positive strain.
机译：具有双峰光活性的碳水化合物共轭硅（IV）酞菁被开发为具有荧光标记和光敏功能的探针，并探索了革兰氏阳性和革兰氏阴性模型的伴随荧光标记和光致失活。麦芽六糖共轭的光探针提供双重读数以区分两组病原体，因为只有革兰氏阳性菌种被灭活，即使它们都被标记为近红外发光。抗生素耐药性没有阻碍光毒性作用，因为即使耐甲氧西林的病原体金黄色葡萄球菌（MRSA）也被完全光灭活。时间分辨共聚焦荧光显微镜分析表明，光探针粘在微生物的外缘上，从而根据革兰氏阴性菌的膜组成解释了其抗性。甘露糖偶联的光探针产生不同的读数，因为它能够标记和灭活革兰氏阳性菌株。

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