• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Chemistry: A European journal >Thiazole Orange Dimers in DNA: Fluorescent Base Substitutions with Hybridization Readout
【24h】

Thiazole Orange Dimers in DNA: Fluorescent Base Substitutions with Hybridization Readout

机译：DNA中的噻唑橙二聚体：具有杂交读数的荧光碱基取代

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

By using (S)-2-amino-1,3-propanediol as a linker, thiazole orange (TO) was incorporated in a dimeric form into DNA. The green fluorescence (=530nm) of the intrastrand TO dimer is quenched, whereas the interstrand TO dimer shows a characteristic redshifted orange emission (=585nm). Steady-state optical spectroscopic methods reveal that the TO dimer fluorescence is independent of the sequential base contexts. Time-resolved pump-probe measurements and excitation spectra reveal the coexistence of conformations, including mainly stacked TO dimers and partially unstacked ones, which yield exciton and excimer contributions to the fluorescence, respectively. The helicity of the DNA framework distorts the excitonic coupling. In particular, the interstrand TO dimer could be regarded as an excitonically interacting base pair with fluorescence readout for DNA hybridization. Finally, the use of this fluorescent readout was representatively demonstrated in molecular beacons.
机译：通过使用（S）-2-氨基-1,3-丙二醇作为接头，噻唑橙（TO）以二聚体形式掺入DNA。链内TO二聚体的绿色荧光（= 530nm）被淬灭，而链间TO二聚体显示特征性的红移橙色发射（= 585nm）。稳态光谱法表明，TO二聚体荧光独立于顺序的碱基环境。时间分辨的泵浦探针测量和激发光谱揭示了构象的共存，包括主要堆叠的TO二聚体和部分未堆叠的二聚体，分别产生了激子和准分子对荧光的贡献。 DNA框架的螺旋结构扭曲了激子耦合。特别地，链间TO二聚体可以被认为是具有荧光读数的激子相互作用的碱基对，用于DNA杂交。最后，在分子信标中代表性地证明了这种荧光读数的使用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号