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首页> 外文期刊>Chemistry: A European journal >Activity Enhancement of G-Quadruplex/Hemin DNAzyme by Flanking d(CCC)
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Activity Enhancement of G-Quadruplex/Hemin DNAzyme by Flanking d(CCC)

机译：侧翼d（CCC）增强G-四链体/血红素DNA酶的活性

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摘要

G-quadruplex (G4)/hemin DNAzymes have been extensively applied in bioanalysis and molecular devices. However, their catalytic activity is still much lower than that of proteinous enzymes. The G4/hemin DNAzyme activity is correlated with the G4 conformations and the solution conditions. However, little is known about the effect of the flanking sequences on the activity, though they are important parts of G4s. Here, we report sequences containing d(CCC), flanked on both ends of the G4-core sequences remarkably enhance their DNAzyme activity. By using circular dichroism and UV-visible spectroscopy, the d(CCC) flanking sequences were demonstrated to improve the hemin binding affinity to G4s instead of increasing the parallel G4 formation, which might explain the enhanced DNAzyme activity. Meanwhile, the increased hemin binding ability promoted the degradation of hemin within the DNAzyme by H2O2. Furthermore, the DNAzyme with d(CCC) flanking sequences showed strong tolerance to pH value changes, which makes it more suitable for applications requiring wide pH conditions. The results highlight the influence of the flanking sequences on the DNAzyme activity and provide insightful information for the design of highly active DNAzymes.
机译：G-quadruplex（G4）/ hemin DNAzymes已广泛应用于生物分析和分子装置。但是，它们的催化活性仍远低于蛋白质酶。 G4 / hemin DNAzyme活性与G4构象和溶液条件相关。但是，关于侧翼序列对活性的影响知之甚少，尽管它们是G4的重要组成部分。在这里，我们报告含有d（CCC）的序列，其侧翼位于G4核心序列的两端，显着增强了其DNAzyme活性。通过使用圆二色性和紫外可见光谱，d（CCC）侧翼序列被证明可以改善对G4的血红素结合亲和力，而不是增加平行的G4形成，这可能解释了增强的DNAzyme活性。同时，增加的血红素结合能力促进了H2O2降解DNAzyme中的血红素。此外，具有d（CCC）侧翼序列的DNAzyme对pH值变化表现出较强的耐受性，这使其更适合需要宽pH条件的应用。结果突出了侧翼序列对DNAzyme活性的影响，并为高活性DNAzyme的设计提供了深刻的信息。

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