• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Chemistry: A European journal >Ferrous Iron Binding Key to Mms6 Magnetite Biomineralisation: A Mechanistic Study to Understand Magnetite Formation Using pH Titration and NMR Spectroscopy
【24h】

Ferrous Iron Binding Key to Mms6 Magnetite Biomineralisation: A Mechanistic Study to Understand Magnetite Formation Using pH Titration and NMR Spectroscopy

机译：亚铁结合键的Mms6磁铁矿的生物矿化：借助pH滴定法和NMR光谱法了解磁铁矿形成的机理研究

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Formation of magnetite nanocrystals by magnetotactic bacteria is controlled by specific proteins which regulate the particles' nucleation and growth. One such protein is Mms6. This small, amphiphilic protein can self-assemble and bind ferric ions to aid in magnetite formation. To understand the role of Mms6 during in vitro iron oxide precipitation we have performed in situ pH titrations. We find Mms6 has little effect during ferric salt precipitation, but exerts greatest influence during the incorporation of ferrous ions and conversion of this salt to mixed-valence iron minerals, suggesting Mms6 has a hitherto unrecorded ferrous iron interacting property which promotes the formation of magnetite in ferrous-rich solutions. We show ferrous binding to the DEEVE motif within the C-terminal region of Mms6 by NMR spectroscopy, and model these binding events using molecular simulations. We conclude that Mms6 functions as a magnetite nucleating protein under conditions where ferrous ions predominate.
机译：趋磁细菌形成磁铁矿纳米晶体的过程受到特定蛋白质的控制，这些蛋白质调节颗粒的成核和生长。一种这样的蛋白质是Mms6。这种小的两亲蛋白可以自我组装并结合铁离子，从而有助于磁铁矿的形成。为了了解Mms6在体外氧化铁沉淀过程中的作用，我们进行了原位pH滴定。我们发现Mms6在铁盐沉淀过程中影响很小，但是在亚铁离子的掺入和该盐转化为混合价铁矿物质的过程中影响最大，表明Mms6具有迄今未记录的亚铁相互作用特性，促进了铁矿中磁铁矿的形成。富含铁的解决方案。我们通过NMR光谱显示亚铁结合到Mms6的C端区域内的DEEVE主题，并使用分子模拟对这些结合事件进行建模。我们得出结论，在亚铁离子占主导地位的条件下，Mms6可作为磁铁矿成核蛋白。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号