Two-photon excitation and time-resolved fluorescence: 1. The proper response function for analysing single-photon counting experiments

Habenicht A.; Hjelm J.; Mukhtar E.; Bergstrom F.; Johansson LBA.

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Two-photon excitation and time-resolved fluorescence: 1. The proper response function for analysing single-photon counting experiments

机译：双光子激发和时间分辨荧光：1.用于分析单光子计数实验的适当响应函数

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An accurate instrumental response function is needed to deconvolute fluorescence data obtained by time-correlated single-photon counting (TCSPC) upon multi-photon excitation. Hitherto the response function was obtained by measuring Rayleigh scattering (RS) from colloidal solutions, as is also used in one-photon excited fluorescence. We show that hyper Rayleigh scattering (HRS) provides a better choice for deconvolution of fluorescence decays, as obtained by TCSPC and two-photon excitation (TPE). The one- and two-photon response functions were monitored as RS and HRS from colloidal gold particles at 800 and 400 nm, respectively. (C) 2002 Published by Elsevier Science B.V. [References: 23]

机译：需要精确的仪器响应函数来对通过多光子激发时与时间相关的单光子计数（TCSPC）获得的荧光数据进行去卷积。迄今为止，响应函数是通过测量胶体溶液的瑞利散射（RS）获得的，这也用于单光子激发荧光中。我们表明，超瑞利散射（HRS）为荧光衰减的解卷积提供了更好的选择，如通过TCSPC和双光子激发（TPE）获得的。分别从800和400 nm的胶体金颗粒以RS和HRS监测一光子和两光子响应函数。（C）2002由Elsevier Science B.V.出版[参考文献：23]

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《Chemical Physics Letters》 |2002年第6期|共9页
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Habenicht A.; Hjelm J.; Mukhtar E.; Bergstrom F.; Johansson LBA.;
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1. Two-photon excitation and time-resolved fluorescence: 1. The proper response function for analysing single-photon counting experiments [J] . Habenicht A., Hjelm J., Mukhtar E., Chemical Physics Letters . 2002,第5a6期

机译：双光子激发和时间分辨荧光：1.用于分析单光子计数实验的适当响应函数
2. Two-photon excitation and time-resolved fluorescence: 1. The proper response function for analysing single-photon counting experiments [J] . Habenicht A., Hjelm J., Mukhtar E., Chemical Physics Letters . 2002,第5a6期

机译：双光子激发和时间分辨荧光：1.用于分析单光子计数实验的适当响应函数
3. Two-photon excited fluorescence depolarisation experiments: II. The proper response function for analysing TCSPC data [J] . Ryderfors L, Mukhtar E, Johansson LBA Chemical Physics Letters . 2005,第1a3期

机译：双光子激发荧光去极化实验：II。用于分析TCSPC数据的适当响应功能
4. A space- and time-resolved single-photon counting detector for fluorescence microscopy and spectroscopy [C] . X. Michalet, O.H.W. Siegmund, J.V. Vallerga, Ultrasensitive and Single-Molecule Detection Technologies; Progress in Biomedical Optics and Imaging; vol.7 no.15 . 2006

机译：空间和时间分辨的单光子计数检测器，用于荧光显微镜和光谱学
5. Two-Photon Excitation, Fluorescence Microscopy, and Quantitative Measurement of Two-Photon Absorption Cross Sections [D] . DeArmond, Fredrick Michael. 2017

机译：双光子励磁，荧光显微镜和两光炎吸收横截面的定量测量
6. Fluorescence Instrument Response Standards in Two-Photon Time-Resolved Spectroscopy [O] . RAFAL LUCHOWSKI, MARIUSZ SZABELSKI, PABAK SARKAR, -1

机译：荧光仪器响应标准的双光子时间分辨光谱
7. Scanning-less wide-field single-photon counting device for fluorescence intensity, lifetime and time-resolved anisotropy imaging microscopy [O] . Spitz J. -A, Yasukuni R, Sandeau N, 2008

机译：用于荧光强度，寿命和时间分辨各向异性成像显微镜的免扫描广域单光子计数装置
8. Rapid Identification of Bacteria Using Time-Resolved Fluorescence and Fluorescence Excitation Spectral Methods [R] . Brahma, S. K., Baek, M. P., Gaskill, D., 1985

机译：利用时间分辨荧光和荧光激发光谱法快速鉴定细菌

Two-photon excitation and time-resolved fluorescence: 1. The proper response function for analysing single-photon counting experiments

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