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首页> 外文期刊>Current Biology: CB >Protein Delivery to Vacuole Requires SAND Protein-Dependent Rab GTPase Conversion for MVB-Vacuole Fusion
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Protein Delivery to Vacuole Requires SAND Protein-Dependent Rab GTPase Conversion for MVB-Vacuole Fusion

机译：蛋白质输送到液泡需要MVB-液泡融合的SAND蛋白依赖性Rab GTPase转化

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摘要

Plasma-membrane proteins such as ligand-binding receptor kinases, ion channels, or nutrient transporters are turned over by targeting to a lytic compartment lysosome or vacuole for degradation. After their internalization, these proteins arrive at an early endosome, which then matures into a late endosome with intraluminal vesicles (multivesicular body, MVB) before fusing with the lysosome/vacuole in animals or yeast [1, 2]. The endosomal maturation step involves a SAND family protein mediating Rab5-to-Rab7 GTPase conversion [3]. Vacuolar trafficking is much less well understood in plants [4-6]. Here we analyze the role of the single-copy SAND gene of Arabidopsis. In contrast to its animal or yeast counterpart, Arabidopsis SAND protein is not required for early-to-late endosomal maturation, although its role in mediating Rab5-to-Rab7 conversion is conserved. Instead, Arabidopsis SAND protein is essential for the subsequent fusion of MVBs with the vacuole. The inability of sand mutant to mediate MVB-vacuole fusion is not caused by the continued Rab5 activity but rather reflects the failure to activate Rab7. In conclusion, regarding the endosomal passage of cargo proteins for degradation, a major difference between plants and nonplant organisms might result from the relative timing of endosomal maturation and SAND-dependent Rab GTPase conversion as a prerequisite for the fusion of late endosomes/MVBs with the lysosome/vacuole.
机译：血浆膜蛋白（例如配体结合受体激酶，离子通道或营养转运蛋白）通过靶向裂解室溶酶体或液泡进行降解而被翻转。它们被内化后，到达早期的内体，然后与管腔内囊泡（多囊体，MVB）成熟为晚期的内体，然后与动物或酵母中的溶酶体/真空融合[1，2]。内体成熟步骤涉及介导Rab5-to-Rab7 GTPase转化的SAND家族蛋白[3]。植物中对叶片的贩运知之甚少[4-6]。在这里，我们分析拟南芥单拷贝SAND基因的作用。与它的动物或酵母对应物相比，拟南芥SAND蛋白不需要早晚内体成熟，尽管它在介导Rab5-Rab7转化中的作用是保守的。相反，拟南芥SAND蛋白对于随后的MVB与液泡融合至关重要。沙突变体不能介导MVB-真空融合不是由持续的Rab5活性引起的，而是反映了激活Rab7的失败。总之，关于货物蛋白质的内体传代以进行降解，植物和非植物生物之间的主要差异可能是由于内体成熟的相对时机和依赖于SAND的Rab GTPase转化，这是后期内体/ MVBs与内含子融合的前提。溶酶体/真空。

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