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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >INTERACTIONS OF VIRAL MATRIX PROTEIN AND NUCLEOPROTEIN WITH THE HOST CELL CYTOSKELETAL ACTIN IN INFLUENZA VIRAL INFECTION
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INTERACTIONS OF VIRAL MATRIX PROTEIN AND NUCLEOPROTEIN WITH THE HOST CELL CYTOSKELETAL ACTIN IN INFLUENZA VIRAL INFECTION

机译：流感病毒感染中病毒基质蛋白和核蛋白与宿主细胞骨架素的相互作用

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摘要

To understand the role of host cell cytoskeleton in regulating the assembly and budding of the externally enveloped viruses, we have studied the interactions between the cytoskeleton and viral proteins in the influenza A virus-infected Madin-Darby canine kidney (MDCK) cells, employing immunofluorescence microscopy and biochemical techniques, The Triton X-100-insoluble cytoskeletons prepared from the infected cells contained at least two viral proteins, viz, matrix protein (MP) and nucleoprotein (NP), the quantities of which considerably reduced (at least by 60%) upon including 1 M sodium chloride or 2.5 M urea in Triton X-100 (2%), Also, an extraction of these cytoskeletons in presence of the reagents that solubilized the cytoskeletal actin resulted in a release of actin together with NP and MP, Besides, these viral proteins were observed to codistribute with actin in both the cytochalasin E-treated and untreated infected cells, These results demonstrate that NP and MP ionically interact with cytoskeletal actin in the influenza virus-infected MDCK cells. [References: 32]
机译：为了了解宿主细胞骨架在调节外部包膜病毒的装配和萌芽中的作用，我们使用免疫荧光技术研究了甲型流感病毒感染的麦丁-达比犬肾（MDCK）细胞中细胞骨架与病毒蛋白之间的相互作用。显微镜和生化技术，从被感染的细胞制备的Triton X-100不溶性细胞骨架中至少含有两种病毒蛋白，即病毒蛋白，基质蛋白（MP）和核蛋白（NP），其数量大大减少（至少减少了60％ ）在Triton X-100中加入1 M氯化钠或2.5 M尿素（2％）后，在溶解细胞骨架肌动蛋白的试剂存在下提取这些细胞骨架会导致肌动蛋白与NP和MP一起释放，此外，观察到这些病毒蛋白在细胞松弛素E处理和未处理的感染细胞中均与肌动蛋白共分布，这些结果表明NP和MP在离子ct与流感病毒感染的MDCK细胞中的细胞骨架肌动蛋白结合。 [参考：32]

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