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首页> 外文期刊>Current Science: A Fortnightly Journal of Research >The gut-associated Klebsiella sp of the apple snail produces multiple polysaccharide degrading enzymes
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The gut-associated Klebsiella sp of the apple snail produces multiple polysaccharide degrading enzymes

机译：苹果蜗牛的肠道相关克雷伯菌属产生多种多糖降解酶

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摘要

Pila globosa, an edible variety of apple snail is a common inhabitant of lentic ecosystem and feeds on plant detritus. The tissue extract of gastrointestinal tract from Pila globosa demonstrated the presence of carboxymethyl cellulase, xylanase, alginate lyase and pectinase activity. Culture-dependent method was used to isolate carboxymethyl cellulose (CMC) degrading bacteria from the gastrointestinal tract of apple snail. Morphologically identical colonies were obtained on M9 gelrite plates containing CMC as carbon source. One such bacterial isolate was purified by streaking and designated as strain PG-1. Bacterial strain PG-1 degraded CMC, pectin, starch and alginate. The 16S rDNA sequence of strain PG1 was 99% identical to Klebsiella oxytoca. Phylogenetic analysis using maximum likelihood revealed the clustering of strain PG1 with the clade belonging to Klebsiella oxytoca type strain.
机译：Pila globosa，一种可食用的苹果蜗牛，是扁豆生态系统的常见种群，以植物碎屑为食。球形毛果胃肠道组织提取物证明了羧甲基纤维素酶，木聚糖酶，藻酸盐裂解酶和果胶酶活性的存在。采用依赖于培养的方法从苹果蜗牛的胃肠道中分离出降解羧甲基纤维素（CMC）的细菌。在含有CMC作为碳源的M9胶凝板上获得了形态上相同的菌落。通过划线纯化一种这样的细菌分离物，并将其命名为菌株PG-1。细菌菌株PG-1降解了CMC，果胶，淀粉和藻酸盐。 PG1菌株的16S rDNA序列与产酸克雷伯菌有99％的同一性。用最大似然法进行的系统进化分析表明，PG1菌株与催产克雷伯氏菌类型菌株的进化枝聚集。

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