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Molecular Characterization of Aromatic Species of the Genus Lippia from the Colombian Neotropics

机译:来自哥伦比亚新​​热带地区的Lippia属的芳香物种的分子表征

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摘要

The genus Lippia (Verbenaceae) includes about 200 species mainly distributed in the Neotropical region. In Colombia, situated in northern South America, Lippia origanoides, Lippia alba, Lippia citriodora and Lippia dulcis have the potential to becomecommercial crops because of their essentials oils. Nevertheless, these species, and the genus as a whole, have been poorly studied from the taxonomical and genetical points of view. We are carrying out molecular characterization of these four species inColombia. Our aim is to study genetic structure in natural versus human-managed populations, which also differ in their mode of propagation (natural propagation versus human-induced clonal propagation, respectively). For doing this, we have carried out apilot study to look for intra- and interspecific polymorphisms in four regions of the chloroplast DNA (cpDNA) (rpsl4-psaB, petA-psbE, trnL-trnF and trnL intron), the internal trasncribed spacer of the ribosomal DNA (ITS) and 50 ISSR loci. Here, we report the main results of this pilot study of DNA polymorphisms and discuss their utility for population genetic studies and future DNA bar-coding initiatives.
机译:Lippia(马鞭草科)属包括约200种,主要分布在新热带地区。在位于南美北部的哥伦比亚,由于其精油,Ligpia origanoides,Lippa alba,Litpia citriodora和Lippia dulcis可能成为商业作物。然而,从分类学和遗传学的角度出发,对这些物种以及整个属的研究很少。我们正在哥伦比亚对这四个物种进行分子鉴定。我们的目标是研究自然种群和人类管理种群的遗传结构,这些种群的繁殖方式也不同(分别为自然繁殖与人为克隆繁殖)。为此,我们进行了apilot研究,以寻找叶绿体DNA(cpDNA)的内部转录间隔子(cpsl4-psaB,petA-psbE,trnL-trnF和trnL内含子)的四个区域内的种内和种间多态性。核糖体DNA(ITS)和50个ISSR位点。在这里,我们报告了DNA多态性的这项初步研究的主要结果,并讨论了其在人群遗传研究和未来DNA条码计划中的效用。

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