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首页> 外文期刊>Acta Horticulturae >Effect of Paclobutrazol, Pluronic-F 68 and Phloroglucinol on Micropropagation of Aloe vera
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Effect of Paclobutrazol, Pluronic-F 68 and Phloroglucinol on Micropropagation of Aloe vera

机译:多效唑,Pluronic-F 68和间苯三酚对芦荟微繁的影响

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摘要

Aloe vera, an important medicinal plant of Liliaceae family, is known as 'wand of heaven' or 'doctor in a pot' as it has more than 200 nutrients which are used in curing different diseases. Indian government is promoting Aloe farming in dry and semi dry areas of India as it requires less water for its growth. Micropropagation is an effective way to provide quality planting material as conventional methods are slow and labor intensive. Sterilized vertical sections were dissected and cultured on MS medium with 2 mg/L BA and 0.18 mg/L IAA. Shoot Initiation started in 12 days. Multiple shoots (8-12 per explant) were observed, which were separated and cultured on six different media including NAA (0.1 mg/L), IBA (0.1 mg/L), IAA (0.1 mg/L), 1.0 mg/L phloroglucinol, 1.0 mg/L pluronic-F 68 and 0.5 mg/L paclobutrazol. It was observed that paclobutrazol enhanced rooting in Aloe vera shoot cultures. Around 12 healthy roots measuring nine cm long were observed in paclobutrazol medium whereas in pluronic- F68 containing medium, maximum of 4 roots per shoot of average 8 cm length obtained. Phloroglucinol medium exhibited three roots per shoot of average 4 cm length. Interestingly on paclobutrazol we obtained shoots with thin skinned fleshy leaves of light green color with high gel quantity of as compared to plants regenerated in other media and field grown plants. These plants showed more number of offshoots when planted in soil.
机译:芦荟是百合科的重要药用植物,因其具有200多种营养素,可用于治愈各种疾病而被称为“天堂之宝”或“锅里的医生”。印度政府正在促进印度干旱和半干旱地区的芦荟种植,因为其生长所需的水更少。由于传统方法缓慢且劳动强度大,因此微繁是提供优质种植材料的有效方法。解剖灭菌的垂直切片,并在含有2 mg / L BA和0.18 mg / L IAA的MS培养基上培养。拍摄开始在12天内开始。观察到多芽(每个外植体8-12),将其分离并在六种不同培养基上培养,包括NAA(0.1 mg / L),IBA(0.1 mg / L),IAA(0.1 mg / L),1.0 mg / L间苯三酚,1.0 mg / L普卢尼克F 68和0.5 mg / L多效唑。观察到多效唑可增强芦荟芽培养物中的生根。在多效唑中大约观察到12个健康的根,长9厘米,而在含有pluronic-F68的培养基中,每个枝条平均平均长8厘米,最多可获得4个根。间苯三酚培养基的每根芽平均显示3根长4 cm的根。有趣的是,与在其他培养基和田间种植的植物中再生的植物相比,在多效唑中,我们获得了具有浅绿色的浅肤色,多肉的叶片的芽,且凝胶含量高。当种植在土壤中时,这些植物显示出更多的分支。

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