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首页> 外文期刊>Acta Horticulturae >Construction of Libraries of 'Micro-Tom' Tomato Mutations Induced by Heavy-Ion Bombardment
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Construction of Libraries of 'Micro-Tom' Tomato Mutations Induced by Heavy-Ion Bombardment

机译：重离子轰击诱导的“微汤姆”番茄突变库的构建

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Fruit setting, development and ripening are complex, genetically programmed processes. Identifying the factors that control these processes is important for understanding the mechanisms of reproductive development. We induced mutations in the tomato cultivar 'Micro-Tom' by irradiation with accelerated heavy ions, recently established as an effective method for inducing mutations in plants, and constructed mutation libraries. Screening 2064 MI plants, we found various mutations, including those affecting the sizes of plants, leaves, or fruit, and one conferring a broccoli-like inflorescence. To date, we have visually phenotyped 516 M_2 families (five sibs per line) in the field, and found 60 plants differing from the wild type in one or more characteristics. The fruit of one was orange, and the beta-carotene content was increased at the expense of lycopene. These results suggest that such mutation libraries could be powerful tools to explain the reproductive development of plants.
机译：坐果，发育和成熟是复杂的，经过基因编程的过程。确定控制这些过程的因素对于理解生殖发育的机制很重要。我们通过加速重离子辐射诱导番茄品种'Micro-Tom'中的突变，最近建立了该突变体作为诱导植物中突变的有效方法，并构建了突变文库。筛选了2064种MI植物，我们发现了各种突变，包括那些影响植物，叶片或果实大小的突变，以及一种赋予西兰花样花序的突变。迄今为止，我们已经在野外对516个M_2家族（每系5个同胞）进行了表型分析，发现了60种与野生型植物有一个或多个特征的植物。一个的果实为橙色，并且β-胡萝卜素的含量增加了番茄红素的损失。这些结果表明，这种突变库可能是解释植物生殖发育的有力工具。

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