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首页> 外文期刊>Acta Horticulturae >A preliminary investigation on the effect of seed physiological stage, concentration and duration of exposure to calcium hypochlorite on in vitro germinability and seedling development of Phalaenopsis amabilis orchids.
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A preliminary investigation on the effect of seed physiological stage, concentration and duration of exposure to calcium hypochlorite on in vitro germinability and seedling development of Phalaenopsis amabilis orchids.

机译：种子生理期，暴露于次氯酸钙的浓度和持续时间对蝴蝶兰的体外萌发能力和幼苗发育的影响的初步研究。

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摘要

P. amabilis fruits collected at 90, 105 and 120 days after pollination (DAP) and surface treated with 15% calcium hypochlorite (CH) for 30 minutes were compared with seeds treated with 0, 5, 10, or 15% CH for 5, 10, or 15 minutes. Ninety-six percent of the untreated seeds from 90-DAP fruit surface-sterilized with 15% CH produced protocorms within 40 days after sowing (DAS). Seed sterilization with 5% CH and exposure duration greater than 5 minutes significantly reduced protocorm percentage. Seeds treated with 10 or 15% CH showed inhibited protocorm development at all exposure times. Protocorms developed root hairs and shoot primodia by 50 DAS and an average of one leaf and root by 85 DAS after treatment with either 0 or 5% CH. Only 27 and 22% of the seeds developed into protocorms but not roots or shoots following treatment with 10 or 15% CH, respectively. P. amabilis flowers were hand-pollinated, and fruits harvested at 90, 105 and 120 DAP while still green were used for seed developmental analysis. The number of cells per seed was estimated by counting nuclei stained with 4'-6-diamidino-2-phenylindole using confocal microscopy. The germination percentage and number of cells per embryo increased from 14 to 61% and 41 to 66%, respectively, during fruit development from 90 to 120 DAP. Harvesting fruits at 120 DAP while still green and fruit sterilization before plating seeds produced the highest percentage of protocorms. Seed sanitation with more than 5% CH markedly reduced protocorm and seedling development.
机译：将在授粉后（DAP）90、105和120天收集并用15％次氯酸钙（CH）表面处理30分钟的美洲山梨果实与用0、5、10或15％CH处理5的种子进行比较， 10或15分钟。 90％DAP水果中未经处理的种子中有96％用15％CH进行表面灭菌，在播种后40天内产生了原球茎。 5％CH和暴露持续时间大于5分钟的种子灭菌显着降低了原球茎的百分比。用10％或15％CH处理的种子在所有暴露时间均显示出原球茎发育受到抑制。在用0或5％CH处理后，原球茎通过50 DAS发育出根毛并发芽初生，而通过85 DAS则平均形成了一片叶和根。用10％或15％CH处理后，分别只有27％和22％的种子发育为原球茎，而没有根或芽。手动对P. amabilis花进行授粉，并在仍为绿色的情况下以90、105和120 DAP收获的果实用于种子发育分析。通过使用共聚焦显微镜计数用4'-6-二mid基-2-苯基吲哚染色的核来估计每个种子的细胞数。在果实发育从90到120 DAP期间，发芽率和每个胚细胞的数量分别从14％增至61％和41％增至66％。以120 DAP的速度收获果实，同时仍保持绿色，并且在接种种子之前对果实进行杀菌，从而产生了最高比例的原球茎。含5％以上CH的种子卫生显着降低了原球茎和幼苗的发育。

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