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首页> 外文期刊>Acta Horticulturae >Molecular diagnostics in the Mycosphaerella leaf spot disease complex of banana and for Radopholus similis.
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Molecular diagnostics in the Mycosphaerella leaf spot disease complex of banana and for Radopholus similis.

机译：香蕉和香蕉Radopholus similis的霉菌叶斑病复合物的分子诊断。

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摘要

Mycosphaerella leaf spots and nematodes threaten banana cultivation worldwide. The Mycosphaerella disease complex involves three related ascomycetous fungi: Mycosphaerella fijiensis, M. musicola and M. eumusae. The exact distribution of these three species and their disease epidemiology remain unclear, since their symptoms and life cycles are rather similar. Diagnosing these diseases and the respective causal agents is based on the presence of host symptoms and fungal fruiting structures, but is time consuming and not conducive to preventive management. In the present study, we developed rapid and robust species-specific diagnostic tools to detect and quantify M. fijiensis, M. musicola and M. eumusae. Conventional species-specific PCR primers were developed based on the actin gene that detected as little as 100, 1 and 10 pg/μl DNA from, respectively, M. fijiensis, M. musicola and M. eumusae. Furthermore, TaqMan real-time quantitative PCR assays that were developed based on the ?-tubulin gene detected quantities as low as 1 pg/μl DNA of each species from pure cultures and 1.6 pg/μl DNA/mg of M. fijiensis from dry leaf tissue. The efficacy of the tests was validated using naturally infected banana leaves. Similar technology has been used to develop a quantitative PCR assay for the banana burrowing nematode, Radopholus similis, which is currently being validated.
机译：真菌球菌叶斑和线虫威胁着全世界的香蕉种植。霉菌病复合体涉及三种相关的子囊真菌：斐济霉菌，M。musicola和M. eumusae。这三种物种的确切分布及其疾病流行病学仍然不清楚，因为它们的症状和生命周期相当相似。诊断这些疾病和各自的病因是基于宿主症状和真菌结实结构的存在，但是很费时且不利于预防管理。在本研究中，我们开发了快速而强大的物种特定诊断工具来检测和量化斐济分枝杆菌，M。musicola和M. Eumusae。基于肌动蛋白基因开发了常规的物种特异性PCR引物，该肌动蛋白基因分别从斐济分枝杆菌，M。musicola和M. eumusae中检测到低至100、1和10 pg /μlDNA。此外，基于α-微管蛋白基因开发的TaqMan实时定量PCR分析检测到纯培养物中每种菌种的DNA量低至1 pg /μl，而干叶中的斐济分支杆菌的DNA量低至1.6 pg /μlDNA / mg。组织。使用天然感染的香蕉叶验证了测试的有效性。相似的技术已被用于开发香蕉穴居线虫Radopholus similis的定量PCR检测方法，该方法目前正在验证中。

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