Long-term subculture and maintenance of transgenic resistant embryogenic callus in litchi ( Litchi chinensis  Sonn.).

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Long-term subculture and maintenance of transgenic resistant embryogenic callus in litchi ( Litchi chinensis Sonn.).

机译：荔枝转基因抗性胚发生愈伤组织的长期传代培养和维持。

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The effects of subculture method, hygromycin, AgNO₃, inoculation quantity and subculture cycle on the growth of litchi transgenic resistant embryogenic callus (TREC) were studied in this experiment. Meanwhile, the influences of the medium and long-term subculture on the GUS expression of litchi resistant embryogenic callus were carried out. The obtained results showed that litchi TREC could be steadily maintained for a long time when they were alternately subcultured in the MS medium containing 1.0 mg/L 2,4-D, 50 mg/L hygromycin and the MS medium supplemented with 1.0 mg/L 2,4-D, 100 mg/L inositol and 50 mg/L hygromycin.

机译：研究了继代培养方法，潮霉素，AgNO _{3 ，接种量和继代培养周期对荔枝转基因抗性胚发生愈伤组织（TREC）生长的影响。同时，进行了中长期培养对荔枝抗性胚发生愈伤组织GUS表达的影响。获得的结果表明，将荔枝TREC在含有1.0 mg / L 2,4-D，50 mg / L潮霉素的MS培养基和补充有1.0 mg / L的MS培养基中交替传代培养后，可以长期稳定地维持。 2,4-D，100 mg / L肌醇和50 mg / L潮霉素。}

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《Acta Horticulturae》 |2010年第863期|共6页
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2; 4-D; callus; growth; hygromycin B; in vitro culture; myo-inositol; plant growth regulators; silver nitrate; tissue culture; transgenic plants; Litchi chinensis; plants;

机译：2;4-D;愈伤组织;生长;潮霉素B;离体培养;肌醇;植物生长调节剂;硝酸银;组织培养;转基因植物;中华荔枝;植物;

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1. Long-term subculture and maintenance of transgenic resistant embryogenic callus in litchi ( Litchi chinensis Sonn.). [J] . Acta Horticulturae . 2010,第863期

机译：荔枝转基因抗性胚发生愈伤组织的长期传代培养和维持。
2. A simple pollen collection, dehydration, and long-term storage method for litchi (Litchi chinensis Sonn.) [J] . Wang Limin, Wu Jiefang, Chen Jiezhen, Scientia horticulturae . 2015,第Null期

机译：荔枝（荔枝）的简单花粉收集，脱水和长期保存方法
3. Leaf callus induction and suspension culture establishment in lychee (Litchi chinensis Sonn.) cv. Huaizhi [J] . Ma Xin-Ye, Yi Gan-Jun, Huang Xue-Lin, Acta Physiologiae Plantarum . 2009,第2期

机译：荔枝（Litchi chinensis Sonn。）cv。的叶片愈伤组织诱导和悬浮培养的建立。怀直
4. Long-Term Subculture and Maintenance of Transgenic Resistant Embryogenic Callus in Litchi(Litchi chinensis Sonn.) [C] . C.C.Lai, Z.X.Lai, Q.L.Sang, The Third International Conference Symposium on Longan,Lychee and Other Fruit Trees in Sapindaceae Family(第三届国际龙眼，荔枝及其他无患子科果树学术研讨会） . 2008

机译：荔枝（Litchi chinensis Sonn。）转基因抗性胚发生愈伤组织的长期继代培养和维持
5. THE MEGAGAMETOPHYTES OF THE LYCHEE (LITCHI CHINENSIS, SONN.). [D] . MUSTARD, MARGARET JEAN. 1958

机译：荔枝的大型化生植物（美国里奇中国）。
6. The effect of desulfurization on the postharvest quality and sulfite metabolism in pulp of sulfitated Feizixiao Litchi (Litchi chinensis Sonn.) fruits [O] . Tao Luo, Shuangshuang Li, Dongmei Han, 2019

机译：脱硫对硫酸化肥子笑荔枝果实果肉采后品质和亚硫酸盐代谢的影响
7. Desenvolvimento do fruto da lichieira (Litchi chinensis Sonn.) 'Bengal' Fruit development in 'Bengal' litchi (Litchi chinensis Sonn.) [O] . Luiz Carlos Chamhum Salomão, Dalmo Lopes de Siqueira, Marcio Eduardo Canto Pereira 2006

机译：Desenvolvimento do fruto da lichieira（Litchi chinensis sonn。）'Bengal''孟加拉'荔枝果实发育（Litchi chinensis sonn。）

Long-term subculture and maintenance of transgenic resistant embryogenic callus in litchi ( Litchi chinensis Sonn.).

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