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首页> 外文期刊>Acta Horticulturae >Evaluation of the seed germination of two protected species: Lilium pomponium L. and Lilium martagon L.
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Evaluation of the seed germination of two protected species: Lilium pomponium L. and Lilium martagon L.

机译：评价两种受保护物种：绒球百合和and百合。

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摘要

The genus Lilium includes near 100 species widespread in Europe, Asia and North America. Among these L. martagon L. and L. pomponium L. are protected by regional laws in Liguria and Piemonte. L. pomponium is a Ligurian-Provencal endemic species growing, in Italy, only in Western Liguria. L. martagon is widely spread in Europe and Asia but, as a result of intensive collection, is rare in Italy. In the genus Lilium seeds germinate by two different types: 'epigeal' or 'hypogeal'. Within each type, the germination can be 'delayed' or 'immediate' related to seed dormancy (Lighty, 1968; Baranova, 1977; Pelkonen, 2005). It is well known that L. martagon, has a hypogeal germination, but nothing is known about L. pomponium. Our experiments allowed to classify the germination of this species as 'delayed epigeal' germination. L. pomponium seed morphology and viability were analysed. Seeds were stored at 15 degrees C with or without dehydration at 12% RH, prior to storage. Germination pre-treatments consisted of chilling (4 degrees C) for 30 or 60 days or warm temperature (24 degrees C) for 30 or 60 days or combination of them. Seeds were then sown in petri with wet filter paper, at 18+or-1 degrees C in dark. The best germination percentage (70%) was obtained after the pre-treatment combination of warm (30 days) and cold (60 days) stratification. The dehydration before storage did not affect germination potential. Fresh L. martagon seeds were used for in vitro germination experiments. A first sterilization trial with NaOCl 4,8% of free chlorine for 3 min, was compared with a treatment with ethanol 70% followed by NaOCl 4,8% of free chlorine for 20 min; both were rinsed several times with sterile distilled water. Seeds were then placed on a solid MS medium with sucrose (3% w/v), and incubated at 20/10 degrees C with a photoperiod of 12/12h (light/dark). After 4 months it was observed that the second sterilization treatment guaranteed higher sterility and that the germination percentage in vitro reached 97%.
机译：百合属（Lilium）包括近100种，分布在欧洲，亚洲和北美。在这些 L中。 martagon L.和 L。利古里亚和皮埃蒙特（Piemonte）的地方性法律保护绒球。 L。 m 是利古里亚-普罗旺斯地区的特有物种，仅在意大利西部利古里亚地区生长。 L。 martagon 在欧洲和亚洲广泛传播，但由于集约化，在意大利很少见。在百合属中，种子发芽有两种不同类型：“上生的”或“次生的”。在每种类型中，发芽可能与种子休眠相关“延迟”或“立即”（Lighty，1968; Baranova，1977; Pelkonen，2005）。众所周知， L。 martagon ，具有胚芽发芽，但对 L却一无所知。 m 。我们的实验允许将该物种的萌发分类为“延缓的表皮”萌发。 L。分析了m的种子形态和活力。在储存之前，将种子在15℃下在有或没有脱水的条件下在12％RH下保存。萌芽预处理包括冷藏（4摄氏度）30或60天或温暖温度（24摄氏度）30或60天或它们的组合。然后用湿滤纸将种子播种在皮氏培养皿中，温度为18+或-1摄氏度，黑暗中。在温暖（30天）和寒冷（60天）分层的预处理组合后，可获得最佳发芽率（70％）。储存前的脱水不影响发芽潜力。新鲜 L。 martagon 种子用于体外发芽实验。将首次用4.8％NaOCl的游离氯消毒3分钟进行的灭菌试验与先用70％乙醇处理，然后用4.8％NaOCl的游离氯处理20分钟的试验进行比较。两者均用无菌蒸馏水冲洗几次。然后将种子置于含蔗糖（3％w / v）的固体MS培养基上，并在20/10摄氏度下以12 / 12h（亮/暗）的光周期孵育。 4个月后，观察到第二次灭菌处理保证了更高的无菌性，并且体外发芽率达到了97％。

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