Characterization of  Pseudomonas syringae  pv.  actinidiae  populations using different typing techniques.

Mazzaglia A.; Renzi M.; Taratufolo M. C.; Balestra G. M.

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Characterization of Pseudomonas syringae pv. actinidiae populations using different typing techniques.

机译：丁香假单胞菌的鉴定。猕猴桃种群使用不同的打字技术。

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Over the last few years a severe bacterial disease on kiwifruit has been reported in Italy; its causal agent was identified as Pseudomonas syringae pv. actinidiae. For epidemiological purposes, we evaluated the discriminatory power of various PCR-based fingerprinting approaches; profiles were generated by rep-PCR (including also (GTG)5-PCR), IS50-PCR, and RAPD, from a wide collection of Italian, Korean and Japanese strains, to investigate the genetic relationships between them and also with some representative strains of related pathovars. It wasn't possible to find dissimilarities among Italian strains, whilst some were evidenced between these and the Japanese/Korean strains of this pathovar. Also, the other pathovars clustered separately, with the pathovar theae being always more closely related to actinidiae than the others. In general, the results obtained with the different methods were in good agreement, with RAPD showing the highest values of divergence between groups.

机译：在过去的几年中，意大利报道了猕猴桃的一种严重细菌性疾病。其致病菌被鉴定为丁香假单胞菌。猕猴桃。出于流行病学的目的，我们评估了各种基于PCR的指纹识别方法的区分能力;通过rep-PCR（还包括（GTG）5-PCR），IS50-PCR和RAPD，从大量的意大利，韩国和日本菌株中生成了图谱，以研究它们之间以及与某些代表性菌株的遗传关系相关的伤痛者。不可能在意大利毒株之间发现差异，而在这些病原体与日本/韩国毒株之间已发现一些差异。另外，其他病原体单独聚集，病原体 theae 总是比其他病原体更紧密地与 actinidiae 相关。通常，使用不同方法获得的结果非常吻合，其中RAPD显示出组间差异的最大值。

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《Acta Horticulturae》 |2011年第913期|共5页
作者
Mazzaglia A.; Renzi M.; Taratufolo M. C.; Balestra G. M.;
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正文语种 eng
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characterization; kiwifruits; plant diseases; plant pathogenic bacteria; plant pathogens; random amplified polymorphic DNA; Actinidia; Actinidia deliciosa; Pseudomonas syringae pv. actinidiae; Developed Countries; European Union Countries; Mediterranean Region; OECD Countries; Southern Europe; Europe; APEC countries; East Asia; Asia; Developing Countries; Threshold Countries; Actinidia; Actinidiaceae; Theales; Ericales; dicotyledons; angiosperms; Spermatophyta; plants; eukaryotes; Pseudomonas syringae; Pseudomonas; Pseudomonadaceae; Pseudomonadales; Gammaproteobacteria; Proteobacteria; Bacteria; bacterium; prokaryotes.;

机译：猕猴桃;植物病;植物病原菌;植物病原体;随机扩增多态DNA;猕猴桃;猕猴桃;丁香假单胞菌PV。猕猴桃;发达国家;欧洲联盟国家;地中海地区;经合组织国家;南欧;欧洲;APEC国家;东亚;亚洲;发展中国家;门槛国家;猕猴桃;猕猴桃科;Theales;Ericales;双子叶类;血管植物;精子;植物;真核生物;丁香假单胞菌;假单胞菌;假单胞菌科;假单胞菌;伽玛变形杆菌;变形杆菌;细菌;细菌;原核生物。;

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专利

1. Characterization of Pseudomonas syringae pv. actinidiae populations using different typing techniques. [J] . Mazzaglia A., Renzi M., Taratufolo M. C., Acta Horticulturae . 2011,第913期

机译：丁香假单胞菌的鉴定。猕猴桃种群使用不同的打字技术。
2. Método de detección de Pseudomonas syringae pv. actinidiae (Psa) en ramas asintomáticas de actinidia↓Método de dete??o de Pseudomonas syringae pv. actinidiae (Psa) em ramos assintomáticos de actinídea [J] . Abelleira Adela, Yebra Aitana Ares, Casal Olga Aguín, Revista de Ciências Agrárias . 2015,第2期

机译：丁香假单胞菌的检测方法。猕猴桃无症状分支中的猕猴桃（Psa）↓丁香假单胞菌PV的检测方法。猕猴桃无症状分支中的猕猴桃（Psa）
3. Highly specific assays to detect isolates of Pseudomonas syringae pv. actinidiae biovar 3 and Pseudomonas syringae pv. actinidifoliorum directly from plant material [J] . Andersen M. T., Templeton M. D., Rees-George J., Fortschritte der Physik . 2018,第5期

机译：高度特异性的测定以检测假单胞菌皂苷PV的分离物。 Actinidiae Biovar 3和Pseudomonas syringae pv。猕猴桃直接来自植物材料
4. Using qPCR to monitor populations of Pseudomonas syringae pv. actinidiae on kiwifruit vines after spray application of Bacstar~(TM) [C] . I.P.S. Pushparajah, T.R. Ryan, L.G. Hawes, Annual Conferenceof the New Zealand Plant Protection Society s . 2014

机译：使用QPCR监测假单胞菌粉末PV的群体。猕猴桃上的猕猴桃葡萄藤葡萄藤，在喷射施用后〜（TM）
5. Strain typing and characterization of sigma54-dependent transcriptional activator mutants in Pseudomonas syringae pv. tomato [D] . Baker, Christina Marie. 2008

机译：丁香假单胞菌PV中sigma54依赖性转录激活突变体的菌株分型和表征。番茄
6. Characterization of Bacteriophages against Pseudomonas Syringae pv. Actinidiae with Potential Use as Natural Antimicrobials in Kiwifruit Plants [O] . Oriana Flores, Julio Retamales, Mauricio Núñez, 2020

机译：对掺入PSSudomonas的噬菌体的特征。猕猴桃潜在用途作为Kiwifruit植物的天然抗微生物
7. Characterization of Pseudomonas syringae pv. actinidiae (Psa) isolated from France and assignment of Psa biovar 4 to a de novo pathovar: Pseudomonas syringae pv. actinidifoliorum pv. nov. [O] . Cunty, Amandine, Poliakoff, F., Rivoal, C., 2015

机译：丁香假单胞菌PV的表征。从法国分离的猕猴桃（Psa），并将Psa biovar 4分配给新的病原体：假单胞菌丁香假单胞菌pv。放线菌十一月

Characterization of Pseudomonas syringae pv. actinidiae populations using different typing techniques.

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