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首页> 外文期刊>Acta Horticulturae >Expression of regeneration competence for adventitious budding, rooting and somatic embryogenesis comparing temperate fruit tree species.
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Expression of regeneration competence for adventitious budding, rooting and somatic embryogenesis comparing temperate fruit tree species.

机译：与温带果树种比较，不定芽，生根和体细胞胚发生的再生能力表达。

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Fruit trees belong to the recalcitrant species to regeneration. Neo-formed shoots, roots or somatic embryos appear after single culture sequences corresponding to successive phases of induction, initiation and finally elongation (roots), development (buds), or expression (somatic embryos) according to the organogenic process engaged. Each of the successive steps required specific culture conditions to control according to the genotypes. A comparison has been made between apple, plum, cherry and relevant trees triggering the commonalities between processes of regeneration. Explants must have reached a sufficient physiological maturity before wounding and submitting them to treatments that will activate the perception capacity of the cells to exogenous and single growth regulator at the highest frequency. A gradient of response to the organogenic process highlights more numerous targeted cells in the proximal areas of sub-apical leaves and main roots. The other areas of the explants are involved with optimizing the regeneration conditions. Several cell layers are targeted simultaneously to organize and form new buds and roots on the expression medium. Adventitious budding and somatic embryogenesis can be sustained along subcultures on the expression medium. Through adapted hormonal balance, it is possible to target the layer origin for regenerating buds and the organ for somatic embryos. According to the different levels in root cells competence, various organogenic programs simultaneously occur. This comparison shows a similar approach of any regeneration process for any genotype of fruit tree, somatic embryogenesis staying genetic dependent.
机译：果树属于顽固的可再生物种。根据所涉及的器官发生过程，新培养的芽，根或体胚出现在单个培养序列之后，分别对应于诱导，起始和最终延伸（根），发育（芽）或表达（体胚）的连续阶段。每个后续步骤都需要特定的培养条件以根据基因型进行控制。比较了苹果，李子，樱桃和引发再生过程之间的共性的相关树木。外植体在受伤之前必须已经达到足够的生理成熟度，然后进行治疗，以激活细胞以最高频率感知外源和单一生长调节剂的能力。对器官发生过程的响应梯度突出显示了在根尖下叶和主根的近端区域中更多的靶向细胞。外植体的其他区域与优化再生条件有关。同时靶向几个细胞层，以在表达培养基上组织并形成新的芽和根。不定芽和体细胞胚发生可以通过在表达培养基上的亚培养来维持。通过适应性荷尔蒙平衡，有可能针对该层的起源来再生芽，而针对器官的器官则针对体细胞胚。根据根细胞能力的不同水平，同时发生各种器官发生程序。该比较显示了对于任何基因型的果树，体细胞胚发生保持遗传依赖性的任何再生过程的相似方法。

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