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首页> 外文期刊>Acta Horticulturae >Micropropagation of Nepeta nuda ssp. nuda (Lamiaceae) - influence of auxins and cytokinins.
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Micropropagation of Nepeta nuda ssp. nuda (Lamiaceae) - influence of auxins and cytokinins.

机译：Nepeta nuda ssp的微繁殖。 nuda（唇形科）-生长素和细胞分裂素的影响。

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摘要

The genus Nepeta (Lamiaceae) comprises more than 250 species of annuals and perennials, most of which growing wild in Central and Southern Europe, Northern Africa, Central and Southern Asia. In this study, Nepeta nuda L. ssp. nuda was collected in Lozen mountain location near Sofia and propagated successfully in vitro on MS medium. Nepeta nuda ssp. nuda has diuretic, anti-spasmodic, anti-asthmatic, antioxidant, as well as sedative properties. During the in vitro growth, the number and length of shoots, callogenesis capability, root formation, and chlorophyll a and b content were determined. The effect of a range of concentrations (0.1-1.0 mg L-1) of cytokinin 6-benzylaminopurine (BA), and auxin, indole-3-butyric acid (IBA), was investigated. Significant stimulation of the shoots number was observed on MS medium supplemented with 0.8 mg L-1 BA in comparison with untreated control plants. All concentrations of BA suppressed root formation, and only high concentrations induced callogenesis. Slight increase in chlorophyll a content was detected after growth on medium supplemented with 1.0 mg L-1 BA. IBA stimulated the root formation and increased the shoots' length at high concentrations (0.7-1.0 mg L-1). This is the first report on in vitro propagated Nepeta nuda ssp. nuda through a direct plant regeneration approach, and the present work offers an effective alternative method for propagation and preservation of this important medicinal plant.
机译：荆芥属（菊科）包括250多种一年生和多年生植物，其中大多数在中欧和南欧，北非，中亚和南亚野生生长。在这项研究中，Nepeta nuda L. ssp。 nuda收集在索非亚附近的Lozen山区，并在MS培养基上成功繁殖。荆芥属nuda具有利尿，抗痉挛，抗哮喘，抗氧化剂以及镇静作用。在体外生长过程中，确定了芽的数量和长度，愈伤能力，根的形成以及叶绿素a和b的含量。研究了一系列浓度（0.1-1.0 mg L -1 ）的细胞分裂素6-苄氨基嘌呤（BA）和植物生长素吲哚-3-丁酸（IBA）的影响。与未处理的对照植物相比，在添加0.8 mg L -1 BA的MS培养基上观察到明显的芽数刺激。所有浓度的BA抑制根形成，只有高浓度的BA诱导愈伤组织。在补充有1.0 mg L -1 BA的培养基上生长后，检测到叶绿素a含量略有增加。在高浓度（0.7-1.0 mg L -1 ）下，IBA刺激了根的形成并增加了芽的长度。这是关于体外繁殖的荆芥（Nepeta nuda ssp）的第一份报告。 nuda通过直接的植物再生方法，目前的工作为这种重要药用植物的繁殖和保存提供了有效的替代方法。

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