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首页> 外文期刊>Acta Horticulturae >Molecular marker-assisted introgression of wild asparagus species genome into the cultivated Asparagus officinalis L.
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Molecular marker-assisted introgression of wild asparagus species genome into the cultivated Asparagus officinalis L.

机译：分子标记辅助将野生芦笋种基因组渗入栽培的芦笋。

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摘要

Interspecific hybridization in Asparagus genus is expected to introgress desirable characteristics of wild species (good taste of spears, adaptation to biotic and abiotic stresses) into the cultivated one (A. officinalis L). To this purpose two species spontaneous in the Mediterranean climate were considered: A. maritimus L. Miller, resistant to rust disease (Puccinia asparagi) and tolerant to sa A. acutifolius L., adapted to xerophytic conditions. Besides both species are well known for good taste of their spears. To overcome the sexual incompatibility between A. officinalis and A. acutifolius a bridge-cross "A. officinalis (4n) x A. maritimus (4n)" was necessary. The "three-ways" interspecific hybrid plant obtained (OMA) was crossed to the tetraploide A. officinalis cultivar 'Violetto d'Albenga' and eight BC1 (OMAO) plants were generated. One of these plants gave nine dihaploid (DH) and seven tetraploid androgenetic clones following in vitro anther culture. Dihaploid plants crossed with diploid A. officinalis genotypes gave F1 fertile plants (DO). The objective of this study was the assessment of genomic introgression of A. acutifolius and A. maritimus into progenies derived from backcross to A. officinalis by means of RAPD molecular markers. Results obtained showed that A. acutifolius genome was present in the OMA plant, but most of it was lost in the genotypes derived both from backcrosses with A. officinalis and anther culture. Moreover, the genome introgression of A. maritimus was not demonstrated because none of the markers used discriminated this species from A. officinalis.
机译：芦笋属的种间杂交有望将野生物种的理想特征（长矛的美味，对生物和非生物胁迫的适应性）渗入培养的一种（ officinalis L ）。为此，考虑了在地中海气候中自发的两个物种：A。 maritimus L. Miller，对铁锈病（ Puccinia asparagi ）有抵抗力，并耐盐; A。 acutifolius L.，适合于旱生条件。此外，这两个物种的矛口都很好。为了克服A之间的性不相容。厚皮和 A。 acutifolius 跨过桥“ A。officinalis （4n）x maritimus （4n）”是必要的。将获得的“三向”种间杂种植物（OMA）与四倍体 1 （OMAO）植物。这些植物之一在体外花药培养后产生了九个二倍体（DH）和七个四倍体雄激素克隆。二倍体植物与二倍体A杂交。基因型产生了F 1 可育植物（DO）。这项研究的目的是评估 A的基因组渗入。 cut 和 A。 maritimus 转化为与 A回交的后代。药用植物的RAPD分子标记。所得结果表明，A。 acutifolius基因组存在于OMA植物中，但大部分丢失于与 A回交的基因型中。药用植物和花药培养。此外，A的基因组基因渗入。没有证明maritimus ，因为所用的标记物均未将其与 A区分开。药用。

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