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首页> 外文期刊>Acta Horticulturae >Evaluation of genetic fidelity of in vitro propagated apple (Malus x domestica Borkh.) rootstock MM 106 using ISSR markers.
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Evaluation of genetic fidelity of in vitro propagated apple (Malus x domestica Borkh.) rootstock MM 106 using ISSR markers.

机译：使用ISSR标记评估离体繁殖的苹果（Malus x domestica Borkh。）砧木MM 106的遗传保真度。

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摘要

True-to-type clonal fidelity is of paramount concern during micropropagation of crop species. Though, micropropagation has found an important role in propagation of apple rootstocks, commercial propagation may be constrained by the occurrence of genetic variations, especially when cultures are maintained in vitro for a long period. To determine genetic fidelity of micropropagated plants of apple rootstock MM 106 multiplied by enhanced axillary branching method, we screened 24 inter simple sequence repeat markers (ISSR). Of these, 11 primers reproducibly generated a total of 107 distinct bands. Apple rootstock Merton 793 and scion 'Jonathon' were analysed along with tissue culture raised progenies of MM 106 as outliers to rule out the possibility that the invariant banding pattern was on account of inefficiency of ISSR primers in detecting variations. The number of bands per primer varied from 5 to 9 with an average of 9.72 scorable bands per primer. Among 83 bands amplified in micropropagated plants of MM 106, 78 bands were monomorphic (93.9%) and 5 bands (6.02%) were polymorphic. The results showed that ISSR markers can be used to gain rapid and precise information about the genetic stability of micropropagated apple rootstock MM 106 and corroborated the fact that the cultures multiplied through pre-existing meristems (axillary branching) may also show genetic instability especially upon extended in vitro cultivation.
机译：真正的克隆保真度是农作物微繁殖过程中最重要的问题。尽管微繁殖已发现在苹果砧木繁殖中的重要作用，但是商业繁殖可能会受到遗传变异的限制，特别是当培养物在体外长期保存时。为了确定苹果砧木MM 106的微繁植物的遗传保真度乘以增强的腋生分支方法，我们筛选了24个简单的序列重复标记（ISSR）。其中，11个引物可重复生成总共107条不同的条带。对苹果砧木Merton 793和接穗“ Jonathon”以及MM 106的组织培养后代进行了分析，以排除异常条带模式是由于ISSR引物检测变异的效率低下的可能性。每个引物的条带数目从5到9不等，每个引物平均有9.72个可评分的条带。在MM 106微繁殖植物中扩增的83条带中，有78条带是单态的（93.9％），有5条带（6.02％）是多态的。结果表明，ISSR标记可用于获得有关微繁殖苹果砧木MM 106遗传稳定性的快速准确信息，并证实以下事实：通过预先存在的分生组织繁殖的培养物（腋生分支）也可能显示遗传不稳定性，尤其是在延长时体外培养。

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