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首页> 外文期刊>Acta Horticulturae >Preliminary results on the use of Melia azedarach fruit extracts as antimicrobial agents in the micropropagation of Zantedeschia.
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Preliminary results on the use of Melia azedarach fruit extracts as antimicrobial agents in the micropropagation of Zantedeschia.

机译：of子花果实提取物在马蹄莲微繁殖中用作抗菌剂的初步结果。

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Bacterial contamination can be a great problem for in vitro plant tissue culture. The present study investigates the antimicrobial activity of fruit extracts of Melia azedarach on some bacterial isolates ('spot agar test') and contaminants of in vitro shoots of Zantedeschia aethiopica, and the influence of the extracts on shoot growth. The shoots were grown on a proliferation medium (ZP) consisting of basal MS composition and BA 3 mg/L. Bacterial strains were isolated from various micropropagated plants, and identified as Sphingomonas paucimobilis (Sp), Bacillus circulans (Bc), Kocuria kristinae (Kk), and A17 (under identification). Forty gram fruit pulp and skin of Melia were homogenized in 100 ml distilled water (PA extract) or 50% ethanol (Et-OH) solution (PE extract). PEE was obtained from PE by Et-OH evaporation. Variable amounts (0, 1, 5, 10 and 20%) of PE and PEE fruit extracts and Et-OH solution were added to ZP, and their influence on shoot growth was evaluated. Zantedeschia shoots were inoculated with different bacterial strains and grown on standard ZP medium to allow bacteria spread. Shoots were treated with 10% PEE and subcultured again on ZP. Shoot homogenates were then processed for bacteria counts (colony forming units, cfu, g-1 fresh tissue). PE and PEE at 1% positively affected shoot growth; however, growth decreased as the extract amount increased. No growth, and severe yellowing occurred with 20% PEE, and even shoot death with PE at the same level. PEE was the most active extract, having a bactericidal activity against all the bacterial isolates, except Sp, while the Et-OH solution had no antimicrobial activity. PEE also strongly reduced Bc and Kk development in contaminated Zantedeschia shoots. These results suggest that fruit extracts of M. azedarach represent a promising alternative to antibiotics as antimicrobial treatments for bacteria-contaminated in vitro grown ornamental plants.
机译：对于体外植物组织培养，细菌污染可能是一个大问题。本研究调查了苦ia子果实提取物对某些细菌分离物（“琼脂试验”）和离体马鞭草马齿Z的污染物的抗菌活性，以及​​提取物对枝条生长的影响。将芽生长在由基础MS组成和BA 3 mg / L组成的增殖培养基（ZP）上。从各种微繁殖的植物中分离出细菌菌株，并将其鉴定为鲍氏鞘氨醇单胞菌（Spphomoomos paucimobilis，Sp），圆形芽孢杆菌（Bacillus circulans，Bc），Kuricia kristinae（Kk）和A17（待鉴定）。在100 ml蒸馏水（PA提取物）或50％乙醇（Et-OH）溶液（PE提取物）中匀浆40克Melia的果肉和皮肤。通过Et-OH蒸发从PE中获得PEE。将不同量（0％，1、5％，10％和20％）的PE和PEE水果提取物以及Et-OH溶液添加到ZP中，并评估它们对枝条生长的影响。用不同的细菌菌株接种马蹄莲芽，并在标准ZP培养基上生长以使细菌传播。将芽用10％PEE处理并再次在ZP上继代培养。然后将枝条匀浆进行细菌计数（菌落形成单位，cfu，g -1 新鲜组织）。 PE和PEE的1％对芽生长有积极影响;但是，随着提取物量的增加，生长减少。 PEE浓度为20％时，无生长，严重发黄，甚至在相同水平的PE下也可导致枝条死亡。 PEE是活性最高的提取物，对除Sp以外的所有细菌分离物均具有杀菌活性，而Et-OH溶液则没有抗菌活性。 PEE还可以大大减少受污染的马蹄莲芽中Bc和Kk的发育。这些结果表明，作为一种受细菌污染的体外观赏植物的抗微生物治疗剂，棉铃虫的果实提取物可以替代抗生素。

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