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首页> 外文期刊>Acta Horticulturae >Preparation of Antiserum to Recombinant Coat Protein for Detecting Strawberry Mild Yellow Edge Virus
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Preparation of Antiserum to Recombinant Coat Protein for Detecting Strawberry Mild Yellow Edge Virus

机译：用于检测草莓轻度黄缘病毒的重组外壳蛋白抗血清的制备

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摘要

Strawberry mild yellow edge virus (SMYEV) is one of the most economically important viral pathogens infecting strawberries worldwide. Immunodiagnostics is a rapid and sensitive method for detecting viruses. Preparation of antiserum against recombinantcoat protein (CP) has been used for some viruses. In this paper, the full length CP gene of SMYEV, which consisted of 729 bp and encoded 242 amino acid residues, was isolated from the SMYEV isolate SY05, and then cloned into the prokaryotic expression vector pGEX-6P-l. The recombinant prokaryotic expression vector carrying the SMYEV CP gene was introduced into Escherichia coli and the fused CP protein was expressed in E. coli cells treated with IPTG The antiserum was produced after the rabbit was immunized with the purified CP protein. Two methods, ELISA and RT-PCR, were compared for detecting SMYEV, and the results show that ELISA was as not sensitive as RT-PCR.
机译：草莓轻度黄缘病毒（SMYEV）是感染全世界草莓的最经济重要的病毒病原体之一。免疫诊断是检测病毒的快速而敏感的方法。针对重组病毒（CP）的抗血清的制备已用于某些病毒。本文从SMYEV分离株SY05中分离了SMYEV全长CP基因，该序列由729 bp组成，编码242个氨基酸残基，然后克隆到原核表达载体pGEX-6P-1中。将携带SMYEV CP基因的重组原核表达载体导入大肠杆菌，并在用IPTG处理的大肠杆菌细胞中表达融合的CP蛋白。用纯化的CP蛋白免疫兔子后产生抗血清。比较了ELISA和RT-PCR这两种检测SMYEV的方法，结果表明ELISA与RT-PCR一样不灵敏。

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