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首页> 外文期刊>Acta Horticulturae >Analysis of flavonoid-biosynthesis gene expression in the flowers of major lily cultivars.
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Analysis of flavonoid-biosynthesis gene expression in the flowers of major lily cultivars.

机译：主要百合品种花中黄酮类生物合成基因表达分析。

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摘要

Flavonoids biosynthesis pathway mostly determines the flower colour pheno-types in plants. This study was carried out to determine the presence and expression rates of the flavonoids biosynthesis-related genes in major lily cultivars. Primers of major colour genes were prepared by comparing the homologous genes of other species like sorghum, onion, and rice, and used for RT- PCR of eight lily cultivars. The results of transcriptional rate analyses showed that, in pink-flowering lily cultivars like, 'Pirax', red-flowering cultivar 'Atika', yellow-flowering cultivar 'Gironde', and white cultivar 'Siberia', all the genes tested were expressed. However, F3'H and DFR genes were not expressed in yellow-flowering cultivar 'Yelloween'. In white-flowering cultivars 'Samarinde' 'Richmond' and 'White Elegance', gene expressions varied among cultivars, especially in the genes CHS, F3H and ANS. We assume that the results on the presence and/or expression rates of the major flavonoid-biosynthesis genes shall be useful in the hybridization and transformation breeding in lilies for flower colour modification.CT III International Symposium on the Genus Lilium, Zhangzhou, Fujian, China.
机译：类黄酮的生物合成途径主要决定植物中花色的表型。进行这项研究以确定主要百合品种中黄酮类生物合成相关基因的存在和表达率。主要颜色基因的引物是通过比较其他物种（例如高粱，洋葱和水稻）的同源基因而制备的，并用于8个百合品种的RT-PCR。转录速率分析的结果表明，在粉色花朵的百合品种中，如“ Pirax”，红色花朵的品种“ Atika”，黄色花朵的品种“ Gironde”和白色品种的“西伯利亚”，都表达了所有测试的基因。 。然而，F3'H和DFR基因在黄色开花品种'Yelloween'中不表达。在开白花的品种“ Samarinde”，“ Richmond”和“ White Elegance”中，基因表达在品种之间变化，尤其是在CHS，F3H和ANS基因中。我们认为主要黄酮类生物合成基因的存在和/或表达率的结果将对百合进行花色修饰的杂交和转化育种有用。CTIII国际百合属研讨会，福建漳州。

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