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首页> 外文期刊>Acta Horticulturae >Growth and morphogenesis of Protea cynaroides L. microshoots cultured under light-emitting diodes (LEDs).
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Growth and morphogenesis of Protea cynaroides L. microshoots cultured under light-emitting diodes (LEDs).

机译：在发光二极管（LED）下培养的Protea cynaroides L. microshoots的生长和形态发生。

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Growth and morphogenesis of Protea cynaroides L. explants in vitro are relatively slow. Besides being essential for photosynthesis, light quality also plays an important role in plant morphogenesis. In this study, the effects of light quality on the growth of P. cynaroides apical shoots cultured under light-emitting diodes (LEDs) were investigated. Three light treatments were used: conventional cool white fluorescent lamps (FL) (control), far-red (FR) LEDs (770 nm), and red+far-red (R+FR) (1:1 spectral energy) LEDs (640+770 nm). Two types of growth media were used: half-strength Murashige and Skoog (MS) medium supplemented with either 0.1 mg L-1 naphthalene acetic acid (NAA) or 2 mg L-1 zeatin and 0.5 mg L-1 indole-3-acetic acid (IAA). After 35 days in culture, chlorophyll content (SPAD value) of apical shoots was lowest in explants cultured in growth medium containing 2 mg L-1 zeatin and 0.5 mg L-1 IAA, and irradiated by FR LEDs. Significantly higher chlorophyll content was detected in explants cultured under R+FR LEDs on medium containing 0.1 mg L-1 NAA, which was similar to those grown on medium containing 2 mg L-1 zeatin and 0.5 mg L-1 IAA under white fluorescent lamps. Under white light, the bud weight of apical buds cultured on media supplemented with 2 mg L-1 zeatin and 0.5 mg L-1 IAA was significantly higher than the other treatments. The highest number of buds was observed on shoots irradiated by white light and R+FR LEDs, cultured on media containing 2 mg L-1 zeatin and 0.5 mg L-1, and 0.1 mg L-1 NAA, respectively. Explants irradiated by FR LEDs on medium containing 0.1 mg L-1 NAA produced the least number of buds. Results suggest that irradiation of P. cynaroides explants by LEDs containing red light induces the growth of new buds in the absence of zeatin.
机译：拟南芥外植体的生长和形态发生相对较慢。除了对光合作用至关重要外，光质在植物形态发生中也起着重要作用。在这项研究中，研究了光质量对在发光二极管（LED）下培养的P. cynaroides顶芽生长的影响。使用了三种光处理：常规的冷白色荧光灯（FL）（对照），远红（FR）LED（770 nm）和红+远红（R + FR）（1：1光谱能量）LED（ 640 + 770 nm）。使用两种类型的生长培养基：半强度Murashige和Skoog（MS）培养基，添加0.1 mg L -1 萘乙酸（NAA）或2 mg L -1 玉米蛋白和0.5 mg L -1 吲哚-3-乙酸（IAA）。培养35天后，在含有2 mg L -1 玉米素和0.5 mg L -1 的生长培养基中培养的外植体中，顶芽的叶绿素含量（SPAD值）最低。 IAA，并由FR LED照射。在含有0.1 mg L -1 NAA的培养基上，在R + FR LED下培养的外植体中，叶绿素含量显着升高，这与在含有2 mg L -1的培养基上生长的植株相似。白色荧光灯下加入sup>玉米素和0.5 mg L -1 IAA。在白光下，在添加了2 mg L -1 玉米素和0.5 mg L -1 IAA的培养基上培养的顶芽的芽重显着高于其他处理。在包含2 mg L -1 玉米素和0.5 mg L -1 的培养基上培养的白光和R + FR LED照射的芽上观察到最多的芽，和0.1 mg L -1 NAA。在含有0.1 mg L -1 NAA的培养基上，用FR LED照射的植株产生的芽数最少。结果表明，在没有玉米素的情况下，含有红光的LED照射的C. cynaroides外植体诱导了新芽的生长。

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