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首页> 外文期刊>Acta Horticulturae >Micropropagation of Czech Apple Cultivars
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Micropropagation of Czech Apple Cultivars

机译：捷克苹果品种的微繁

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The objective of this study was to investigate micropropagation protocols for use with three apple cultivars (‘James Grieve Compact’, ‘Jarka’ and ‘Mivibe’) bred in the Czech Republic. Apple shoot tips (5 to 10 mm in length) were established in vitro using 0.15“/o HgCl_2 as sterilization solution. Contamination rate, survival and development of shoots from excised shoot tips were analyzed after sterilization. For the three cultivars, the effect of three growth regulators 1, 2 and 4 mg L~(-1) BAP (6-benzylaminopurine), 0.5 and 1 mg L~(-1) TDZ or 10 mg L~(-1) 2iP in basal MS medium on proliferation, callus formation and shoot morphology is shown. Values of proliferation rate varied between 1.1 and 3.6. Generally, the highest rate was obtained for cultivar ‘Jarka’ that produced 3.6 new shoots on MS medium containing 4 mg L~(-1) BAP. The lowest number of newly formed shoots (1.1) was noted for cultivars ‘James Grieve Compact’ and ‘Mivibe’ on MS medium with low concentration of BAP (1 mg L~(-1)). In vitro plants of ‘James Grieve Compact’ were of poor quality and developed symptoms of yellowing or chlorosis of leaves in later subcultures. It was possible to maintain actively growing in vitro cultures of ‘James Grieve Compact’ on all tested multiplication media only for a limited number of subcultures (usually, 8-9 subcultures; total culture period about 10 months), after which they either ceased to proliferate and multiply or eventually turned yellow-hrown and subsequently died out. Theeffects of indole-3-butyric acid, indole-3-acetic acid or naphtalene acetic acid in concentration 1 mg L~(-1) on root induction were tested in MS medium for cultivars ‘Jarka’ and ‘Mivibe’. Rooting was induced for both genotypes tested. However, theroot induction was relatively low from 0 to 44% of in vitro rooted plants.
机译：这项研究的目的是研究在捷克共和国饲养的三种苹果品种（“詹姆斯·格里夫·紧凑”，“贾尔卡”和“米维贝”）使用的微繁殖方案。使用0.15“ / o HgCl_2作为灭菌溶液，在体外建立了苹果芽梢（长5至10 mm）。灭菌后，分析切下的芽梢的芽的污染率，存活率和发育。对于这三个品种，三个生长调节剂1、2和4 mg L〜（-1）BAP（6-苄氨基嘌呤），0.5和1 mg L〜（-1）TDZ或10 mg L〜（-1）的作用显示了在基础MS培养基中2iP对增殖，愈伤组织形成和枝条形态的影响。增殖率的值在1.1和3.6之间变化。通常，最高的品种是'Jarka'，在含有4 mg L〜（-1）BAP的MS培养基上产生3.6个新芽。在低BAP浓度（1 mg L〜（-1））的MS培养基上，品种'James Grieve Compact'和'Mivibe'的新形成芽的数量最少（1.1）。 “ James Grieve Compact”的体外植株质量较差，在随后的亚培养中出现叶片泛黄或褪绿的症状。仅在有限数量的亚培养物中（通常是8-9次亚培养;总培养期约10个月），才有可能在所有测试的繁殖培养基上维持“ James Grieve Compact”的体外培养物的活跃生长，然后停止培养。繁殖并繁殖或最终变成黄褐色，随后消失。在MS培养基中测试了'Jarka'和'Mivibe'品种的浓度为1 mg L〜（-1）的吲哚-3-丁酸，吲哚-3-乙酸或萘萘乙酸对根诱导的影响。两种测试的基因型都可以生根。但是，离体生根植物的根诱导相对较低，从0％到44％。

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