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首页> 外文期刊>Acta Horticulturae >Plant Abiotic Stress Tolerance Analysis in Cauliflower Using a Curd Micropropagation System
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Plant Abiotic Stress Tolerance Analysis in Cauliflower Using a Curd Micropropagation System

机译:凝乳微繁殖系统分析花椰菜中的植物非生物胁迫耐受性

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摘要

An effective protocol for cauliflower micropropagation was optimised and developed which enabled the production of tens of thousands of cauliflower microshoots from one cauliflower curd. The large number of microshoots that can be produced per cultureunit facilitates the use of this protocol to analyse both the physiological and molecular components of abiotic stress tolerance. The protocol was used for cauliflower cold tolerance analysis and it was demonstrated that low temperature acclimation increased the cold tolerance of explants. The effect of two additives used with the cauliflower culture media on cold tolerance were evaluated. ABA signillcantly decreased both cold tolerance in acclimated and non-acclimated cauliflower microshoots whilst molybdenum had a highly positive effect on cold tolerance of cauliflower microshoots. Moreover, molybdenum had the capacity to increase the cold tolerance of cauliflower microshoots without low temperature treatment. This is the first study that confirmedthis feature of molybdenum and it is believed that this finding could have an application in the field.
机译:优化和开发了一种有效的花椰菜微繁殖方案,可以从一种花椰菜豆腐生产数以万计的花椰菜微枝。每个培养单位可以产生大量的微芽,这有助于使用该方案来分析非生物胁迫耐受性的生理和分子成分。该方案用于花椰菜的耐寒性分析,并证明低温驯化增加了外植体的耐寒性。评估了两种与花椰菜培养基一起使用的添加剂对耐寒性的影响。 ABA显着降低了适应性和非适应性花椰菜微枝的耐寒性,而钼对花椰菜微枝的耐寒性具有高度积极的影响。此外,钼具有在不进行低温处理的情况下提高花椰菜微枝耐寒性的能力。这是第一个证实了钼的这一特征的研究,并且相信这一发现可以在该领域中得到应用。

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