...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta Horticulturae >Establishment of Cotyledon Explants Regeneration System in Wild Watermelon
【24h】

Establishment of Cotyledon Explants Regeneration System in Wild Watermelon

机译：野生西瓜子叶外植体再生体系的建立

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Wild watermelon is ideal rootstock for cultivated watermelon grafting because of its graft compatibility; tolerance to environmental stresses, and less effect on the fruit quality of the scion. However, it is hard to acquire the excellent wild watermelon genotype with multiple resistances through traditional breeding programs. Genetic transformation is an efficient approach for genetic improvement. In the study, we analyzed the effect of plant growth regulators on the cotyledon regeneration efficiency with the aim of establishing aenetic transformation system for wild watermelon. Wild watermelon (Citrullus lanatus var. citroides) seeds were germinated under aseptic conditions. Cotyledons were cut from five day seedlings and cultured on MS medium supplemented with different plant growth regulators. The results showed that explants cultured in MS + BA (1.0 mg/L) achieved the highest regeneration rate, and 6.3 shoots were regenerated from each explant on an average. Adventitious shoots were able to elongate on MS medium with KT concentrations ranging from 0.1 to 0.3 mg/L. On an average 62% adventitious shoots were successfully rooted on MS medium supplemented with 0.05 mg/L NAA. Rooted seedlings were successfully acclimatized and gradually hardened-off to green-house conditions and subsequently cultivated in soil with a mean survival rate of 80%. The cotyledon regeneration system for wild watermelon was successfully established in the study, which is needed for genetic transformation of wild watermelon in the future.
机译：野生西瓜因其嫁接相容性而成为栽培西瓜嫁接的理想砧木。耐环境胁迫，对接穗果实品质的影响较小。但是，通过传统育种程序很难获得具有多重抗性的优良野生西瓜基因型。遗传转化是遗传改良的有效途径。在研究中，我们分析了植物生长调节剂对子叶再生效率的影响，目的是建立野生西瓜的无性转化体系。野生西瓜种子（Citrullus lanatus var。citroides）种子在无菌条件下发芽。从五天的幼苗中切下子叶，并在补充有不同植物生长调节剂的MS培养基上培养。结果表明，在MS + BA（1.0 mg / L）中培养的外植体再生率最高，平均每个外植体再生6.3枝。不定芽能够在KT浓度为0.1至0.3 mg / L的MS培养基上伸长。平均而言，不定芽平均有62％成功地生根于补充有0.05 mg / L NAA的MS培养基上。生根的幼苗已成功地适应环境，并逐渐变硬为温室条件，随后在土壤中耕种，平均成活率为80％。本研究成功建立了野生西瓜子叶再生体系，是今后野生西瓜遗传转化所需要的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号