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首页> 外文期刊>Acta Horticulturae >Microspore Culture in Eleven Cultivars of Loquat
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Microspore Culture in Eleven Cultivars of Loquat

机译：Lo 11个品种的小孢子培养

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In plant breeding, microspore embryogenesis via isolated microspore culture is increasing as a method to obtain in a single-step doubled haploids and homozygosity. In this study, isolated microspore culture of eleven cultivars of loquat (Eriobotrya japonica (Thunb.) Lindl.) has been carried out. An analysis of anthers from different flower bud sizes, has been carried out, to identify and to select the flower bud size with anthers containing the highest quantity of vacuolated microspores. After a pre-treatment of the panicles at 4°C in dark for 4 days, flower buds were sterilized and microspores were isolated and cultured in two different media (P and MB). Moreover, only the isolated microspores cultured in P Medium were subjected to two additionalthermal shocks: cold shock (1 h at -20°C) and heat shock (7 days at 33°C). All the cultures were placed in a growth cabinet at 26±1°C in the dark for 30 days and later placed in the light. Samples from cultures were processed for microscopy analysisat specific times. Observations on microspore development were carried out by fluorescence and light microscopy. The sporophytic development of isolated microspores in culture has been confirmed by the presence in the cultures of binucleated with two equal-size vegetative-type nuclei that had just started their sporophytic pathway, multinucleated, pollen-derived multicellular structures, and calli. The results are an advancement towards the knowledge of pollen embryogenesis in loquat.
机译：在植物育种中，通过单步获得双倍单倍体和纯合性的方法，通过分离的小孢子培养产生的小孢子胚胎发生在增加。在这项研究中，已对11个of品种（Eriobotrya japonica（Thunb。）Lindl。）进行了分离的小孢子培养。已经对来自不同花芽大小的花药进行了分析，以鉴定和选择花芽大小，其中花药含有最高量的空泡小孢子。在黑暗中于4°C下对穗进行预处理4天后，将花芽灭菌，分离出小孢子并在两种不同的培养基（P和MB）中培养。此外，仅在P培养基中培养的分离出的小孢子受到了两次额外的热冲击：冷冲击（在-20°C时1 h）和热冲击（在33°C时7天）。将所有培养物在黑暗中于26±1°C的生长箱中放置30天，然后置于光线下。来自培养物的样品在特定时间进行显微镜分析。通过荧光和光学显微镜观察小孢子发育。分离的小孢子在孢子体中的孢子发育已被双核培养物中存在，该双核具有两个刚刚开始其孢子性途径的相等大小的营养型核，多核，花粉衍生的多细胞结构和愈伤组织。结果为towards花粉胚胎发生的认识提供了一个进步。

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