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首页> 外文期刊>Acta Horticulturae >The Genetic Determinants of Symptoms Induction by Pepino Mosaic Virus in Tomato
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The Genetic Determinants of Symptoms Induction by Pepino Mosaic Virus in Tomato

机译：Pepino花叶病毒在番茄中诱发症状的遗传决定因素

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The Pepino mosaic vims (PepMV) is one of the most important pathogens infecting tomato crops worldwide. Four major genotypes have been identified so far: Peruvian (LP), European (EU), Chilean 1 (CHI) and Chilean 2 (CH2). In Poland isolates representing the EU and CH2 genotypes have been found. The isolates belonging to the CH2 genotype induce a wide range of symptoms from very mild mosaic or even a lack of symptoms, through severe yellowing, up to necrosis of leaves. Therefore, the characterization of different genetic variants of PepMV is important to clarify processes of disease emergence caused by this virus. The necrotic and yellowing pathotypes representing the Chilean 2 genotype have been extensively studied. Development of infectious clones facilitates manipulation of the viral genome by site-directed mutagenesis. The studies performed made it possible to identify the viral genetic determinants responsible for the induction of different symptoms on infected plants. The introduction of singlenucleotide substitutions in particular positions of triple gene block 3 (TGB3) resulted in changes of symptoms on tomato plants. Moreover, the mutations affect significantly viral accumulation, which has been measured by real-time RT-PCR. Single nucleotide substitutions introduced to the genome of genetically different European isolates also had an effect on biological properties and accumulation of the virus. Identified mutations in the viral genome are responsible for the development of symptoms andvirus accumulation, and may play an important role during infection.
机译：Pepino花叶病毒（PepMV）是感染全世界番茄作物的最重要病原体之一。迄今为止，已鉴定出四种主要基因型：秘鲁（LP），欧洲（EU），智利1（CHI）和智利2（CH2）。在波兰，已发现代表欧盟和CH2基因型的分离株。属于CH2基因型的分离物会引起各种症状，从非常轻微的花叶病，甚至没有症状，到严重的泛黄直至叶坏死。因此，表征PepMV的不同遗传变异对于阐明由该病毒引起的疾病出现过程很重要。代表智利2基因型的坏死和泛黄病型已被广泛研究。感染性克隆的发展有助于通过定点诱变操作病毒基因组。进行的研究使鉴定引起感染植物不同症状的病毒遗传决定因素成为可能。在三重基因区块3（TGB3）的特定位置引入单核苷酸取代导致番茄植株出现症状变化。此外，这些突变会显着影响病毒的积累，这已通过实时RT-PCR测定。引入遗传上不同的欧洲分离株的基因组中的单核苷酸取代也对病毒的生物学特性和积累有影响。病毒基因组中已鉴定的突变是导致症状发展和病毒积累的原因，并且可能在感染过程中发挥重要作用。

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